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Abstract：
Blastocystis sp. is a prevalent protistan parasite found globally in the gastrointestinal tract of humans and various animals. This review aims to elucidate the advancements in research on axenic isolation techniques for Blastocystis sp. and their diverse applications. Axenic isolation, involving the culture and isolation of Blastocystis sp. free from any other organisms, necessitates the application of specific media and a series of axenic treatment methods. These methods encompass antibiotic treatment, monoclonal culture, differential centrifugation, density gradient separation, micromanipulation and the combined use of culture media. Critical factors influencing axenic isolation effectiveness include medium composition, culture temperature, medium characteristics, antibiotic type and dosage and the subtype (ST) of Blastocystis sp. Applications of axenic isolation encompass exploring pathogenicity, karyotype and ST analysis, immunoassay, characterization of surface chemical structure and lipid composition and understanding drug treatment effects. This review serves as a valuable reference for clinicians and scientists in selecting appropriate axenic isolation methods.
摘要：
囊胚。是在人类和各种动物的胃肠道中发现的普遍的protistan寄生虫。这篇综述旨在阐明囊胚的轴性分离技术的研究进展。以及它们多样化的应用。轴性隔离，涉及囊胚的培养和分离。不受任何其他生物的影响，需要应用特定的培养基和一系列的无菌治疗方法。这些方法包括抗生素治疗，单克隆培养，差速离心,密度梯度分离，显微操作和培养基的联合使用。影响无菌隔离效果的关键因素包括培养基成分，培养温度,中等特征，抗生素的类型和剂量以及囊胚菌的亚型（ST）。无菌分离的应用包括探索致病性，核型和ST分析，免疫测定，表征表面化学结构和脂质组成，了解药物治疗效果。这篇综述为临床医生和科学家选择合适的轴性分离方法提供了有价值的参考。
