Abstract：
OBJECTIVE: Runx2 and osteocalcin have pivotal roles in bone homeostasis. Polymorphism of these two genes could alter the function of osteoblasts and consequently bone mineral density (BMD). Attempts to understand the relationship between these polymorphisms and BMD in postmenopausal women across a variety of populations have yielded inconsistent results. This meta-analysis seeks to define the relationship between these polymorphisms with BMD in postmenopausal women.
METHODS: Eligible studies were identified from three electronic databases. Data were extracted from the eligible studies (4 studies on Runx2 and 6 studies on osteocalcin), and associations of Runx2 T > C and osteocalcin HindIII polymorphisms with BMD in postmenopausal women were assessed using standard difference in means (SDM) and 95% confidence intervals (CI) as statistical measures.
RESULTS: A significant difference in the lumbar spine (LS) BMD in postmenopausal women was observed between the TT and CC homozygotes for the Runx2 T > C (SDM = -0.445, p-value = 0.034). The mutant genotypes (CC) showed significantly lower LS BMD in comparison to wild type genotypes under recessive model of genetic analysis (TC + TT vs. CC: SDM = -0.451, p-value = 0.032). For osteocalcin, HindIII polymorphism, the mutant genotypes (HH) was associated with significantly higher BMD for both LS and femoral neck (FN) than the wild type (hh) homozygotes (SDM = 0.152, p-value = 0.008 and SDM = 0.139, p-value = 0.016 for LS and FN, respectively). There was no association between total hip (TH) BMD and the osteocalcin HindIII polymorphism.
CONCLUSIONS: Runx2 T > C and osteocalcin HindIII polymorphisms influence the level of BMD in postmenopausal women and may be used as predictive markers of osteoporosis.
METHODS: Eligible studies were identified from three electronic databases. Data were extracted from the eligible studies (4 studies on Runx2 and 6 studies on osteocalcin), and associations of Runx2 T > C and osteocalcin HindIII polymorphisms with BMD in postmenopausal women were assessed using standard difference in means (SDM) and 95% confidence intervals (CI) as statistical measures.
RESULTS: A significant difference in the lumbar spine (LS) BMD in postmenopausal women was observed between the TT and CC homozygotes for the Runx2 T > C (SDM = -0.445, p-value = 0.034). The mutant genotypes (CC) showed significantly lower LS BMD in comparison to wild type genotypes under recessive model of genetic analysis (TC + TT vs. CC: SDM = -0.451, p-value = 0.032). For osteocalcin, HindIII polymorphism, the mutant genotypes (HH) was associated with significantly higher BMD for both LS and femoral neck (FN) than the wild type (hh) homozygotes (SDM = 0.152, p-value = 0.008 and SDM = 0.139, p-value = 0.016 for LS and FN, respectively). There was no association between total hip (TH) BMD and the osteocalcin HindIII polymorphism.
CONCLUSIONS: Runx2 T > C and osteocalcin HindIII polymorphisms influence the level of BMD in postmenopausal women and may be used as predictive markers of osteoporosis.
摘要：
目的：Runx2和骨钙蛋白在骨稳态中起关键作用。这两个基因的多态性可以改变成骨细胞的功能，从而改变骨矿物质密度（BMD）。试图在各种人群中了解这些多态性与绝经后妇女BMD之间的关系，但结果不一致。这项荟萃分析旨在确定这些多态性与绝经后妇女BMD之间的关系。
方法：从三个电子数据库中确定合格的研究。数据来自符合条件的研究（4项关于Runx2的研究和6项关于骨钙蛋白的研究），使用均值标准差(SDM)和95%置信区间(CI)作为统计指标,评估了Runx2T>C和骨钙素HindIII多态性与绝经后女性BMD的相关性.
结果：对于Runx2T>C，在TT和CC纯合子之间观察到绝经后妇女腰椎（LS）BMD的显着差异（SDM=-0.445，p值=0.034）。在隐性遗传分析模型下，与野生型基因型相比，突变基因型（CC）显示出显着较低的LSBMD（TCTTvs.CC:SDM=-0.451,p值=0.032)。对于骨钙蛋白，HindIII多态性,突变基因型（HH）与LS和股骨颈（FN）的BMD明显高于野生型（HH）纯合子（SDM=0.152，p值=0.008和SDM=0.139，p值=0.016LS和FN，分别)。全髋关节（TH）BMD与骨钙蛋白HindIII多态性之间没有关联。
结论：Runx2T>C和骨钙蛋白HindIII多态性影响绝经后妇女骨密度水平，可作为骨质疏松症的预测指标。
方法：从三个电子数据库中确定合格的研究。数据来自符合条件的研究（4项关于Runx2的研究和6项关于骨钙蛋白的研究），使用均值标准差(SDM)和95%置信区间(CI)作为统计指标,评估了Runx2T>C和骨钙素HindIII多态性与绝经后女性BMD的相关性.
结果：对于Runx2T>C，在TT和CC纯合子之间观察到绝经后妇女腰椎（LS）BMD的显着差异（SDM=-0.445，p值=0.034）。在隐性遗传分析模型下，与野生型基因型相比，突变基因型（CC）显示出显着较低的LSBMD（TCTTvs.CC:SDM=-0.451,p值=0.032)。对于骨钙蛋白，HindIII多态性,突变基因型（HH）与LS和股骨颈（FN）的BMD明显高于野生型（HH）纯合子（SDM=0.152，p值=0.008和SDM=0.139，p值=0.016LS和FN，分别)。全髋关节（TH）BMD与骨钙蛋白HindIII多态性之间没有关联。
结论：Runx2T>C和骨钙蛋白HindIII多态性影响绝经后妇女骨密度水平，可作为骨质疏松症的预测指标。
