RESULTS: This study involved a clinical isolate from the pus of a hospital patient with sebaceous gland abscesses. The clinically isolated strain was identified as C. pyruviciproducens strain WYJY-01. In this study, referring to Koch\'s postulates, we observed the pathological changes of animal models infected by intraperitoneal injection and subcutaneous injection of pure culture of the strain WYJY-01. Furthermore, the strain WYJY-01 was isolated and cultured again from animal models\' subcutaneous abscess drainage fluid. Subsequently, the genomics of the strain WYJY-01 was analyzed. By comparing various gene databases, this study predicted the core secondary metabolite gene cluster of the strain WYJY-01, virulence factor genes carried by prophage, pathogenicity islands, and resistance islands. In addition, the genomes of C. pyruviciproducens strain WYJY-01, ATCC BAA-1742 T, and UMB0763 were analyzed by comparative genomics, and the differential genes of strain WYJY-01 were compared, and their functions were analyzed.
CONCLUSIONS: The findings showed that the strain WYJY-01 had pathogenicity, supplementing the phenotype characteristics of C. pyruviciproducens. Meanwhile, this research revealed the possible molecular mechanism of the pathogenicity of the strain WYJY-01 at the gene level through whole genome sequence analysis, providing a molecular basis for further research.
结果:本研究涉及一名医院皮脂腺脓肿患者脓液的临床分离物。将临床上分离的菌株鉴定为丙酮酸C.productivens菌株WYJY-01。在这项研究中,指的是科赫的假设,我们观察了单纯培养菌株WYJY-01的腹腔注射和皮下注射感染动物模型的病理变化。此外,从动物模型皮下脓肿引流液中分离并再次培养菌株WYJY-01。随后,对WYJY-01菌株进行基因组学分析。通过比较各种基因数据库,本研究预测了菌株WYJY-01的核心次生代谢产物基因簇,噬菌体携带的毒力因子基因,致病性岛,抵抗岛。此外,丙酮酸芽孢杆菌菌株WYJY-01,ATCCBAA-1742T,和UMB0763进行了比较基因组学分析,并对菌株WYJY-01的差异基因进行了比较,并对其功能进行了分析。
结论:结果表明,菌株WYJY-01具有致病性,补充丙酮酸C.的表型特征。同时,本研究通过全基因组序列分析,从基因水平揭示了WYJY-01菌株致病的可能分子机制,为进一步研究提供分子基础。