{Reference Type}: Journal Article
{Title}: Genomic and phenotypic analysis of a novel clinical isolate of Corynebacterium pyruviciproducens.
{Author}: Wang J;Feng J;Jia W;Yuan T;He X;Wu Q;Peng F;Gao W;Yang Z;Tao Y;Li Q;
{Journal}: BMC Microbiol
{Volume}: 23
{Issue}: 1
{Year}: 2023 Dec 6
{Factor}: 4.465
{DOI}: 10.1186/s12866-023-03075-6
{Abstract}: <B>BACKGROUND: </B>Corynebacterium pyruviciproducens is a recently described species of Corynebacterium. There are few reports on the microbiological characteristics of the new species, and there is a lack of reports on the genomic analysis of the species.<BR><B>RESULTS: </B>This study involved a clinical isolate from the pus of a hospital patient with sebaceous gland abscesses. The clinically isolated strain was identified as C. pyruviciproducens strain WYJY-01. In this study, referring to Koch's postulates, we observed the pathological changes of animal models infected by intraperitoneal injection and subcutaneous injection of pure culture of the strain WYJY-01. Furthermore, the strain WYJY-01 was isolated and cultured again from animal models' subcutaneous abscess drainage fluid. Subsequently, the genomics of the strain WYJY-01 was analyzed. By comparing various gene databases, this study predicted the core secondary metabolite gene cluster of the strain WYJY-01, virulence factor genes carried by prophage, pathogenicity islands, and resistance islands. In addition, the genomes of C. pyruviciproducens strain WYJY-01, ATCC BAA-1742 T, and UMB0763 were analyzed by comparative genomics, and the differential genes of strain WYJY-01 were compared, and their functions were analyzed.<BR><B>CONCLUSIONS: </B>The findings showed that the strain WYJY-01 had pathogenicity, supplementing the phenotype characteristics of C. pyruviciproducens. Meanwhile, this research revealed the possible molecular mechanism of the pathogenicity of the strain WYJY-01 at the gene level through whole genome sequence analysis, providing a molecular basis for further research.