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Abstract：
This article provides an overview of microbial host selection, synthetic biology, genome annotation, metabolic modeling, and computational methods for predicting gene essentiality for developing a microbial chassis. This article focuses on lactic acid bacteria (LAB) as a microbial chassis and strategies for genome annotation of the LAB genome. As a case study, Lactococcus lactis is chosen based on its well-established therapeutic applications such as probiotics and oral vaccine development. In this article, we have delineated the strategies for genome annotations of lactic acid bacteria. These strategies also provide insights into streamlining genome reduction without compromising the functionality of the chassis and the potential for minimal genome chassis development. These insights underscore the potential for the development of efficient and sustainable synthetic biology systems using streamlined microbial chassis with minimal genomes.
摘要：
本文概述了微生物宿主选择，合成生物学,基因组注释，代谢建模，和预测开发微生物底盘的基因必要性的计算方法。本文重点介绍了乳酸菌（LAB）作为微生物底盘以及LAB基因组的基因组注释策略。作为一个案例研究，乳酸乳球菌的选择是基于其完善的治疗应用，例如益生菌和口服疫苗开发。在这篇文章中,我们已经描述了乳酸菌基因组注释的策略。这些策略还提供了对简化基因组减少的见解，而不损害底盘的功能和最小基因组底盘开发的潜力。这些见解强调了使用具有最少基因组的流线型微生物底盘开发高效和可持续合成生物学系统的潜力。
