PDF(Pubmed)
Abstract：
UNASSIGNED: The process of decoverslipping is often required in a laboratory to review or examine an older slide which tends to fade over time, making it almost impossible to use it for research or study purposes. The sections then need to be re-stained which can only be done after removing the coverslip. The traditional method of decoverslipping using xylene is a time-consuming process. Various methods have been used in the past; however, none were found to be completely effective. Dry ice, the solid form of carbon dioxide, is an easily available, cheap cooling agent with a low freezing temperature (-78.5°C) which was evaluated for its efficacy in decoverslipping process, as an alternative to xylene.
UNASSIGNED: 64 faded haematoxylin and eosin (H&E)-stained histopathology slides were randomly selected and segregated, according to duration of year, into eight major groups. Each group was further divided into four subgroups according to the time that the slides were subjected for decoverslipping. The slides were placed on dry ice and the time was set. Once the coverslip was removed, the slides were placed in xylene to remove any residual mountant. The tissue sections were evaluated for physical disfigurement followed by re-staining with H&E to check for any change in tissue morphology.
UNASSIGNED: The mean time taken for removal of coverslip using dry ice was 35 seconds.
UNASSIGNED: This technique is easy, fast, and effective, with no tissue loss or compromise in staining quality, thereby preventing xylene toxicity and its effect on the environment.
UNASSIGNED: 64 faded haematoxylin and eosin (H&E)-stained histopathology slides were randomly selected and segregated, according to duration of year, into eight major groups. Each group was further divided into four subgroups according to the time that the slides were subjected for decoverslipping. The slides were placed on dry ice and the time was set. Once the coverslip was removed, the slides were placed in xylene to remove any residual mountant. The tissue sections were evaluated for physical disfigurement followed by re-staining with H&E to check for any change in tissue morphology.
UNASSIGNED: The mean time taken for removal of coverslip using dry ice was 35 seconds.
UNASSIGNED: This technique is easy, fast, and effective, with no tissue loss or compromise in staining quality, thereby preventing xylene toxicity and its effect on the environment.
摘要：
■在实验室中通常需要减滑过程，以检查或检查随着时间的推移而逐渐褪色的旧幻灯片，几乎不可能将其用于研究或学习目的。然后需要重新染色切片，这只能在移除盖玻片后进行。使用二甲苯的减滑的传统方法是耗时的过程。过去已经使用了各种方法；然而，没有发现完全有效。干冰,二氧化碳的固体形式，是一个容易获得的，具有低冷冻温度（-78.5°C）的廉价冷却剂，对其在减滑过程中的功效进行了评估，作为二甲苯的替代品。
■随机选择64个褪色的苏木精和伊红（H＆E）染色的组织病理学载玻片并进行分离，根据一年的持续时间，分为八大组。根据对载玻片进行减滑的时间，将每组进一步分为四个子组。将载玻片置于干冰上并设定时间。一旦盖玻片被移除，将载玻片放置在二甲苯中以去除任何残留的黏附剂。评估组织切片的物理毁容,然后用H&E重新染色以检查组织形态的任何变化。
使用干冰去除盖玻片所花费的平均时间为35秒。
■这项技术很简单，快,而且有效,没有组织损失或染色质量受损，从而防止二甲苯的毒性及其对环境的影响。
■随机选择64个褪色的苏木精和伊红（H＆E）染色的组织病理学载玻片并进行分离，根据一年的持续时间，分为八大组。根据对载玻片进行减滑的时间，将每组进一步分为四个子组。将载玻片置于干冰上并设定时间。一旦盖玻片被移除，将载玻片放置在二甲苯中以去除任何残留的黏附剂。评估组织切片的物理毁容,然后用H&E重新染色以检查组织形态的任何变化。
使用干冰去除盖玻片所花费的平均时间为35秒。
■这项技术很简单，快,而且有效,没有组织损失或染色质量受损，从而防止二甲苯的毒性及其对环境的影响。
