■秸秆资源作为动物饲料的有效利用受到了广泛的关注。这项研究的目的是评估香菇处理是否会改变高地大麦秸秆的化学成分和抗氧化活性,并提高其作为反刍动物饲料的功能价值。
■化学成分,抗氧化能力,在25°C下使用L.sajor-caju进行固态发酵21天后,确定了高地大麦秸秆的代谢组学特征。通过LC-MS鉴定了发酵和未发酵的高原大麦秸秆之间的差异代谢产物,并阐明了鉴定的代谢产物与抗氧化能力之间的关系。
■结果表明,与未经处理的高地大麦秸秆相比,粗蛋白和乙醚提取物含量较高(51.55%和76.43%,分别)在与L.sajor-caju孵育21天后的高地大麦秸秆中,而半纤维素,纤维素,和酸性洗涤剂木质素含量较低(2.48、25.08和45%,分别)。L.sajor-caju处理的总抗氧化能力明显高于未经处理的高地大麦秸秆。总的来说,在L.sajor-caju发酵和未发酵的高地大麦秸秆之间鉴定出600种差异代谢物(301种上调和299种下调)。相关分析结果表明,Fe2+清除能力和总酚含量与总抗氧化能力密切相关。同时,发酵和未发酵的青麦杆之间的差异类黄酮代谢产物主要与抗氧化活性有关,用山奈酚3-木糖葡萄糖苷,等银杏素,Rhoifolin是最具代表性的。
■因此,这项研究表明,L.sajor-caju可以提高高原大麦秸秆的功能价值,显示了L.sajor-caju在提高反刍动物农用秸秆利用率方面的潜力。
UNASSIGNED: The efficient utilization of straw resources as animal feed has gained considerable attention. The objective of this study was to evaluate whether Lentinus sajor-caju treatment alters the chemical composition and antioxidant activity of highland barley straw and enhances its functional value as a ruminant feed.
UNASSIGNED: The chemical composition, antioxidant capacity, and metabolomic profile of highland barley straw were determined after 21 days of solid-state fermentation with L. sajor-caju at 25°C. The differential metabolites between fermented and unfermented highland barley straw were identified by LC-MS and the relationship between the identified metabolites and antioxidant capacity was elucidated.
UNASSIGNED: The results showed that, compared with untreated highland barley straw, the crude protein and ether extract contents were higher (51.55 and 76.43%, respectively) in highland barley straw after 21 days of incubation with L. sajor-caju, whereas the hemicellulose, cellulose, and acid detergent lignin contents were lower (2.48, 25.08, and 45%, respectively). The total antioxidant capacity was significantly higher in L. sajor-caju-treated than in untreated highland barley straw. In total, 600 differential metabolites (301 upregulated and 299 downregulated) were identified between L. sajor-caju-fermented and unfermented highland barley straw. Correlation analysis results showed that Fe2+ scavenging and total phenolic content were strongly correlated with total antioxidant capacity. Meanwhile, the differential flavonoid metabolites between fermented and unfermented highland barley straw were primarily associated with antioxidant activity, with kaempferol 3-xylosylglucoside, isoginkgetin, and rhoifolin being the most representative.
UNASSIGNED: Thus, this study demonstrates that L. sajor-caju could enhance the functional value of highland barley straw, showing the potential of L. sajor-caju for improving the utilization of agricultural straws in ruminants.