Abstract：
A creatively designed novel two-step enhancement technique is presented in which B vitamin molecules are dynamically adsorbed onto the surface of silver nanoparticles by sodium borohydride, followed by local plasmon resonance in the presence of cations (calcium ions), ultimately achieving synergistic chemical and physical enhancement on the same molecule and constructing a \"surface hot spots\" two-step enhancement platform for vitamin detection. The Raman signal of the promoted vitamin molecule is enhanced by nine orders of magnitude. In a subsequent study it was observed that the vitamin B2 molecules were in a near-vertical image on the surface of the silver nanoparticles, which may also contribute to the Raman signal enhancement. Combined with deep learning techniques, the method has been successfully applied to the detection of B vitamins in body fluids. As an accurate, rapid, reproducible, non-invasive, and versatile assay platform, it holds great promise for the intelligent identification of trace B molecules in food, pharmaceuticals, and the human body.
摘要：
提出了一种创造性地设计的新型两步增强技术，其中B族维生素分子被硼氢化钠动态吸附到银纳米颗粒的表面上，随后在阳离子（钙离子）存在下进行局部等离子体共振，最终在同一分子上实现化学和物理协同增强，并构建维生素检测的“表面热点”两步增强平台。促进的维生素分子的拉曼信号增强了9个数量级。在随后的研究中，观察到维生素B2分子在银纳米颗粒的表面上处于接近垂直的图像中，这也可能有助于拉曼信号增强。结合深度学习技术，该方法已成功应用于体液中B族维生素的检测。作为一个准确的，快速,可重复,非侵入性，和多功能测定平台，它为智能识别食品中的痕量B分子带来了巨大的希望，制药，和人体。
