Abstract：
Collagen peptide exhibits a great activity in osteogenic differentiation and wound healing. However, uncontrolled collagen peptide release in bone defects leads to unsatisfactory bone regeneration. In this work, we prepared collagen peptide loaded calcium alginate hydrogel (SA-CP/Ca) derived from Asia carp scales by mixing sodium alginate solution, collagen peptides, calcium carbonate, covalent cross-linking agents N-(3-dimethylaminopropyl)-N\'-ethylcarbodiimide hydrochloride (EDC), and N-hydroxysuccinimide (NHS) in one pot. Physically and chemically double cross-linking realized higher crosslink density, smaller porosity and pore size, and higher energy storage modulus and loss modulus, achieving sustained release of collagen peptides. The release profile is fitted to Keppas-Sahlin model, to find SA-CP/Ca hydrogels are more inclined to release collagen peptides through expansion and degradation. The compatibility and osteogenic ability of SA-CP/Ca are demonstrated in vitro and in vivo.
摘要：
胶原蛋白肽在成骨分化和伤口愈合中表现出很大的活性。然而,骨缺损中不受控制的胶原肽释放导致不令人满意的骨再生。在这项工作中,我们通过混合海藻酸钠溶液制备了来自亚洲鲤鱼鳞片的胶原肽负载海藻酸钙水凝胶（SA-CP/Ca），胶原蛋白肽，碳酸钙，共价交联剂N-(3-二甲基氨基丙基)-N'-乙基碳二亚胺盐酸盐(EDC),和N-羟基琥珀酰亚胺(NHS)在一个锅中。物理和化学双交联实现了更高的交联密度，较小的孔隙率和孔径，和更高的储能模量和损耗模量，实现胶原蛋白肽的持续释放。释放配置文件适合Keppas-Sahlin模型，发现SA-CP/Ca水凝胶更倾向于通过膨胀和降解释放胶原肽。在体外和体内都证明了SA-CP/Ca的相容性和成骨能力。
