骨损伤患病率的上升增加了对微创治疗的需求。微珠水凝胶,以细胞封装而闻名,为骨组织再生提供了一种通用的基质。它们提供生物活性剂,支持细胞生长,促进成骨,帮助骨修复和再生。在这项研究中,我们合成了涂覆有磷酸钙层(m-Sp)的超顺磁性氧化铁纳米颗粒(Sp),实现独特的花状微簇形态。随后,使用滴加凝胶化策略制备含有m-Sp(McSa@m-Sp)的藻酸钠(SA)微珠水凝胶。McSa@m-Sp是可磁性瞄准的,增强交联,控制降解率,并提供强大的抗菌活性。用MC3T3-E1细胞进行的封装研究显示了增强的活力和增殖。这些研究还表明MC3T3-E1细胞中碱性磷酸酶(ALP)活性和矿化显著升高,如茜素红S(ARS)和VonKossa染色所证实,随着McSa@m-Sp微珠水凝胶内胶原蛋白产量的增加。免疫细胞化学(ICC)和基因表达研究支持McSa@m-Sp的骨诱导潜能,显示成骨标志物的表达增加,包括RUNX-2,胶原蛋白-I,骨桥蛋白,和骨钙蛋白。因此,McSa@m-Sp微珠水凝胶为骨组织工程中的多功能支架提供了有希望的策略。
The rising prevalence of bone injuries has increased the demand for minimally invasive treatments. Microbead hydrogels, renowned for cell encapsulation, provide a versatile substrate for bone tissue regeneration. They deliver bioactive agents, support cell growth, and promote osteogenesis, aiding bone repair and regeneration. In this study, we synthesized superparamagnetic iron oxide nanoparticles (Sp) coated with a calcium phosphate layer (m-Sp), achieving a distinctive flower-like micro-cluster morphology. Subsequently, sodium alginate (SA) microbead hydrogels containing m-Sp (McSa@m-Sp) were fabricated using a dropping gelation strategy. McSa@m-Sp is magnetically targetable, enhance cross-linking, control degradation rates, and provide strong antibacterial activity. Encapsulation studies with MC3T3-E1 cells revealed enhanced viability and proliferation. These studies also indicated significantly elevated alkaline phosphatase (ALP) activity and mineralization in MC3T3-E1 cells, as confirmed by Alizarin Red S (ARS) and Von Kossa staining, along with increased collagen production within the McSa@m-Sp microbead hydrogels. Immunocytochemistry (ICC) and gene expression studies supported the osteoinductive potential of McSa@m-Sp, showing increased expression of osteogenic markers including RUNX-2, collagen-I, osteopontin, and osteocalcin. Thus, McSa@m-Sp microbead hydrogels offer a promising strategy for multifunctional scaffolds in bone tissue engineering.