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Abstract：
We have previously produced a toolkit of antibodies, comprising recombinant human antibodies of all but one of the human isotypes, directed against the polcalcin family antigen Phl p 7. In this work, we complete the toolkit of human antibody isotypes with the IgD version of the anti-Phl p 7 monoclonal antibody. We also raised a set of nanobodies against the IgD anti-Phl p 7 antibody and identify and characterize one paratope-specific nanobody. This nanobody also binds to the IgE isotype of this antibody, which shares the same idiotype, and orthosterically inhibits the interaction with Phl p 7. The 2.1 Å resolution X-ray crystal structure of the nanobody in complex with the IgD Fab is described.
摘要：
我们以前生产了一个抗体工具包，包括除一种人类同种型外的所有重组人抗体，针对polcalcin家族抗原Phlp7。在这项工作中,我们使用抗Phlp7单克隆抗体的IgD版本完成了人类抗体同种型工具包。我们还提出了一组针对IgD抗Phlp7抗体的纳米抗体,并鉴定和表征一种互补位特异性纳米抗体。这种纳米抗体还与这种抗体的IgE同种型结合，具有相同的独特型，并在直系上抑制与Phlp7的相互作用。描述了与IgDFab复合的纳米体的2.1µ分辨率X射线晶体结构。
