{Reference Type}: Journal Article
{Title}: Expanding the Anti-Phl p 7 Antibody Toolkit: An Anti-Idiotype Nanobody Inhibitor.
{Author}: Vester SK;Davies AM;Beavil RL;Sandhar BS;Beavil AJ;Gould HJ;Sutton BJ;McDonnell JM;
{Journal}: Antibodies (Basel)
{Volume}: 12
{Issue}: 4
{Year}: 2023 Nov 16
暂无{DOI}: 10.3390/antib12040075
{Abstract}: We have previously produced a toolkit of antibodies, comprising recombinant human antibodies of all but one of the human isotypes, directed against the polcalcin family antigen Phl p 7. In this work, we complete the toolkit of human antibody isotypes with the IgD version of the anti-Phl p 7 monoclonal antibody. We also raised a set of nanobodies against the IgD anti-Phl p 7 antibody and identify and characterize one paratope-specific nanobody. This nanobody also binds to the IgE isotype of this antibody, which shares the same idiotype, and orthosterically inhibits the interaction with Phl p 7. The 2.1 Å resolution X-ray crystal structure of the nanobody in complex with the IgD Fab is described.