%0 Journal Article
%T Expanding the Anti-Phl p 7 Antibody Toolkit: An Anti-Idiotype Nanobody Inhibitor.
%A Vester SK
%A Davies AM
%A Beavil RL
%A Sandhar BS
%A Beavil AJ
%A Gould HJ
%A Sutton BJ
%A McDonnell JM
%J Antibodies (Basel)
%V 12
%N 4
%D 2023 Nov 16
%M 37987253
暂无%R 10.3390/antib12040075
%X We have previously produced a toolkit of antibodies, comprising recombinant human antibodies of all but one of the human isotypes, directed against the polcalcin family antigen Phl p 7. In this work, we complete the toolkit of human antibody isotypes with the IgD version of the anti-Phl p 7 monoclonal antibody. We also raised a set of nanobodies against the IgD anti-Phl p 7 antibody and identify and characterize one paratope-specific nanobody. This nanobody also binds to the IgE isotype of this antibody, which shares the same idiotype, and orthosterically inhibits the interaction with Phl p 7. The 2.1 Å resolution X-ray crystal structure of the nanobody in complex with the IgD Fab is described.