Abstract：
METHODS: Particulate matter (PM) can cause cellular oxidative damage and promote respiratory diseases. It has recently shown that Sargassum horneri ethanol extract (SHE) containing sterols and gallic acid reduces PM-induced oxidative stress in mice lung cells through ROS scavenging and metal chelating. In this study, the role of alveolar macrophages (AMs) is identified that are particularly susceptible to DNA damage due to PM-triggered oxidative stress in lungs of OVA-sensitized mice exposed to PM.
RESULTS: The study scrutinizes if PM exposure causes oxidative DNA damage to AMs differentially depending on their type of polarization. Further, SHE\'s potential is investigated in reducing oxidative DNA damage in polarized AMs and restoring AM polarization in PM-induced allergic airway inflammation. The study discovers that PM triggers prolonged oxidative stress to AMs, leading to lipid peroxidation in them and alveolar epithelial cells. Particularly, AMs are polarized to M2 phenotype (F4/80+ CD206+ ) with enhanced oxidative DNA damage when subject to PM-induced oxidative stress. However, SHE repairs oxidative DNA damage in M1- and M2-polarized AMs and reduces AMs polarization imbalance due to PM exposure.
CONCLUSIONS: These results suggest the possibility of SHE as beneficial foods against PM-induced allergic airway inflammation via suppression of AM dysfunction.
RESULTS: The study scrutinizes if PM exposure causes oxidative DNA damage to AMs differentially depending on their type of polarization. Further, SHE\'s potential is investigated in reducing oxidative DNA damage in polarized AMs and restoring AM polarization in PM-induced allergic airway inflammation. The study discovers that PM triggers prolonged oxidative stress to AMs, leading to lipid peroxidation in them and alveolar epithelial cells. Particularly, AMs are polarized to M2 phenotype (F4/80+ CD206+ ) with enhanced oxidative DNA damage when subject to PM-induced oxidative stress. However, SHE repairs oxidative DNA damage in M1- and M2-polarized AMs and reduces AMs polarization imbalance due to PM exposure.
CONCLUSIONS: These results suggest the possibility of SHE as beneficial foods against PM-induced allergic airway inflammation via suppression of AM dysfunction.
摘要：
方法：颗粒物（PM）可引起细胞氧化损伤并促进呼吸系统疾病。最近表明，含有甾醇和没食子酸的马尾藻乙醇提取物（SHE）通过清除ROS和金属螯合作用降低了PM诱导的小鼠肺细胞氧化应激。在这项研究中,在暴露于PM的OVA致敏小鼠的肺中,由于PM触发的氧化应激,肺泡巨噬细胞(AMs)特别容易受到DNA损伤。
结果：该研究仔细研究了PM暴露是否会导致AM的DNA氧化损伤，这取决于它们的极化类型。Further,研究了SHE在减少极化AM中的氧化DNA损伤和恢复PM诱导的过敏性气道炎症中的AM极化方面的潜力。研究发现PM会引发AM的长期氧化应激，导致它们和肺泡上皮细胞的脂质过氧化。特别是,当受到PM诱导的氧化应激时,AM极化为M2表型(F4/80+CD206+),具有增强的氧化DNA损伤。然而,SHE修复M1和M2极化的AM中的氧化DNA损伤，并减少由于PM暴露引起的AM极化失衡。
结论：这些结果表明，SHE有可能通过抑制AM功能障碍作为抗PM诱导的过敏性气道炎症的有益食物。
结果：该研究仔细研究了PM暴露是否会导致AM的DNA氧化损伤，这取决于它们的极化类型。Further,研究了SHE在减少极化AM中的氧化DNA损伤和恢复PM诱导的过敏性气道炎症中的AM极化方面的潜力。研究发现PM会引发AM的长期氧化应激，导致它们和肺泡上皮细胞的脂质过氧化。特别是,当受到PM诱导的氧化应激时,AM极化为M2表型(F4/80+CD206+),具有增强的氧化DNA损伤。然而,SHE修复M1和M2极化的AM中的氧化DNA损伤，并减少由于PM暴露引起的AM极化失衡。
结论：这些结果表明，SHE有可能通过抑制AM功能障碍作为抗PM诱导的过敏性气道炎症的有益食物。
