Abstract：
Enumeration of bacteriophages by plaque assay requires the mixing of host-specific bacteria with a lytic bacteriophage of interest in a soft agar overlay (top agar) to prevent the spread of viral infection in the medium; the mixture is then spread on a solid bottom agar. An infection of a single lytic phage particle with a bacterium results in the lysis of the host bacterium and the release of new phage progeny. The new phage progeny released from each bacterium will infect/lyse neighboring bacteria to form a \"plaque,\" which is a clear visible area (that can be counted) with the naked eye. If phages are not present in the mixture, the host bacterium will form a \"lawn\" in the soft agar overlay and grow to a stationary phase.
摘要：
通过噬斑测定计数噬菌体需要将宿主特异性细菌与感兴趣的裂解噬菌体在软琼脂覆盖层(顶部琼脂)中混合,以防止病毒感染在培养基中的扩散;然后将混合物铺展在固体底部琼脂上。单个裂解性噬菌体颗粒被细菌感染导致宿主细菌的裂解和新的噬菌体后代的释放。从每个细菌释放的新的噬菌体后代将感染/裂解邻近的细菌以形成“斑块”，“这是一个清晰的肉眼可见的区域（可以计数）。如果混合物中不存在噬菌体，宿主细菌将在软琼脂覆盖层中形成“草坪”，并生长到静止期。
