{Reference Type}: Journal Article
{Title}: Enumeration of Bacteriophages by Plaque Assay.
{Author}: Waturangi DE;
{Journal}: Methods Mol Biol
{Volume}: 2738
{Issue}: 0
{Year}: 2024
暂无{DOI}: 10.1007/978-1-0716-3549-0_9
{Abstract}: Enumeration of bacteriophages by plaque assay requires the mixing of host-specific bacteria with a lytic bacteriophage of interest in a soft agar overlay (top agar) to prevent the spread of viral infection in the medium; the mixture is then spread on a solid bottom agar. An infection of a single lytic phage particle with a bacterium results in the lysis of the host bacterium and the release of new phage progeny. The new phage progeny released from each bacterium will infect/lyse neighboring bacteria to form a "plaque," which is a clear visible area (that can be counted) with the naked eye. If phages are not present in the mixture, the host bacterium will form a "lawn" in the soft agar overlay and grow to a stationary phase.