%0 Journal Article
%T Enumeration of Bacteriophages by Plaque Assay.
%A Waturangi DE
%J Methods Mol Biol
%V 2738
%N 0
%D 2024
%M 37966597
暂无%R 10.1007/978-1-0716-3549-0_9
%X Enumeration of bacteriophages by plaque assay requires the mixing of host-specific bacteria with a lytic bacteriophage of interest in a soft agar overlay (top agar) to prevent the spread of viral infection in the medium; the mixture is then spread on a solid bottom agar. An infection of a single lytic phage particle with a bacterium results in the lysis of the host bacterium and the release of new phage progeny. The new phage progeny released from each bacterium will infect/lyse neighboring bacteria to form a "plaque," which is a clear visible area (that can be counted) with the naked eye. If phages are not present in the mixture, the host bacterium will form a "lawn" in the soft agar overlay and grow to a stationary phase.