PDF(Pubmed)
Abstract：
Wild species field cress (Lepidium campestre) has favorable agronomic traits, making it a good candidate for future development as an oil and catch crop. However, the species is very prone to pod shatter, resulting in severe yield losses. This is one of the important agronomic traits that needs to be improved in order to make this species economically viable. In this study, we cloned the L. campestre INDEHISCENT (LcIND) gene and prepared two LcIND-RNAi constructs with the IND promoter (long 400 bp and short 200 bp) from Arabidopsis. A number of stable transgenic lines were developed and evaluated in terms of pod shatter resistance. The majority of the transgenic lines showed increased resistance to pod shatter compared to the wild type, and this resistance was maintained in four subsequent generations. The downregulation of the LcIND gene by RNAi in the transgenic lines was confirmed by qRT-PCR analysis on T3 lines. Southern blot analysis showed that most of the analyzed lines had a single-copy integration of the transgene, which is desirable for further use. Our results show that it is possible to generate stable transgenic lines with desirable pod shatter resistance by downregulating the LcIND gene using RNAi in field cress, and thus speeding up the domestication process of this wild species.
摘要：
野生水芹（Lepidiumcampestre）具有良好的农艺性状，使其成为未来发展为石油和捕捞作物的良好候选者。然而,这个物种很容易被豆荚粉碎,造成严重的产量损失。这是需要改进以使该物种在经济上可行的重要农艺性状之一。在这项研究中,我们克隆了LcIND基因，并从拟南芥中制备了两个具有IND启动子（长400bp和短200bp）的LcIND-RNAi构建体。开发了许多稳定的转基因品系，并根据豆荚破碎抗性进行了评估。与野生型相比，大多数转基因品系对豆荚破碎的抗性增加，这种抗性在随后的四代中得以维持。通过对T3系的qRT-PCR分析证实了转基因系中RNAi对LcIND基因的下调。Southern印迹分析表明，大多数分析品系具有转基因的单拷贝整合，这是需要进一步使用。我们的结果表明，通过在田间豆瓣菜中使用RNAi下调LcIND基因，可以产生具有理想的豆荚破碎抗性的稳定转基因品系，从而加快了这种野生物种的驯化过程。
