家鸭(Anasplatyrhynchosdomesticus)对大多数高致病性禽流感病毒(HPAIV)感染具有抗性。在这项研究中,我们表征了12小时感染HPAIH5N1病毒(A/duck/India/02CA10/2011/Agartala)的鸭的肺蛋白质组和磷酸蛋白质组,48h,感染后5天。共有2082个蛋白质差异表达,320个磷酸化位点定位到199个磷酸肽,鉴定出对应于129种蛋白质。蛋白质组数据分析的功能注释揭示了RIG-I样受体和Jak-STAT信号通路的激活,这导致了干扰素刺激基因(ISG)表达的诱导。磷酸化蛋白质组数据集的通路分析也证实了RIG-I的激活,Jak-STAT信号,NF-κB信号,和肺组织中的MAPK信号通路。ISG蛋白(STAT1,STAT3,STAT5B,STAT6,IFIT5和PKR)在鸭肺组织中建立了保护性抗病毒免疫应答。Further,蛋白质-蛋白质相互作用网络分析鉴定了AKT1、STAT3、JAK2、RAC1、STAT1、PTPN11、RPS27A、NFKB1和MAPK1是主要的hub蛋白,可能在鸭的疾病进展中起重要作用。一起,蛋白质组和磷酸蛋白质组数据集的功能注释揭示了感染HPAIH5N1病毒的鸭子疾病进展和抗病机制的分子基础。
Domestic ducks (Anas platyrhynchos domesticus) are resistant to most of the highly pathogenic avian influenza virus (HPAIV) infections. In this study, we characterized the lung proteome and phosphoproteome of ducks infected with the HPAI H5N1 virus (A/duck/India/02CA10/2011/Agartala) at 12 h, 48 h, and 5 days post-infection. A total of 2082 proteins were differentially expressed and 320 phosphorylation sites mapping to 199 phosphopeptides, corresponding to 129 proteins were identified. The functional annotation of the proteome data analysis revealed the activation of the RIG-I-like receptor and Jak-STAT signaling pathways, which led to the induction of interferon-stimulated gene (ISG) expression. The pathway analysis of the phosphoproteome datasets also confirmed the activation of RIG-I, Jak-STAT signaling, NF-kappa B signaling, and MAPK signaling pathways in the lung tissues. The induction of ISG proteins (STAT1, STAT3, STAT5B, STAT6, IFIT5, and PKR) established a protective anti-viral immune response in duck lung tissue. Further, the protein-protein interaction network analysis identified proteins like AKT1, STAT3, JAK2, RAC1, STAT1, PTPN11, RPS27A, NFKB1, and MAPK1 as the main hub proteins that might play important roles in disease progression in ducks. Together, the functional annotation of the proteome and phosphoproteome datasets revealed the molecular basis of the disease progression and disease resistance mechanism in ducks infected with the HPAI H5N1 virus.