PDF(Pubmed)
Abstract：
We introduce DEQSeq, a nanopore sequencing approach that rationalizes the selection of favorable genome editing enzymes from directed molecular evolution experiments. With the ability to capture full-length sequences, editing efficiencies, and specificities from thousands of evolved enzymes simultaneously, DEQSeq streamlines the process of identifying the most valuable variants for further study and application. We apply DEQSeq to evolved libraries of Cas12f-ABEs and designer-recombinases, identifying variants with improved properties for future applications. Our results demonstrate that DEQSeq is a powerful tool for accelerating enzyme discovery and advancing genome editing research.
摘要：
我们介绍DEQSeq，一种纳米孔测序方法，可以从定向分子进化实验中合理选择有利的基因组编辑酶。具有捕获全长序列的能力，编辑效率，同时来自数千种进化酶的特异性，DEQSeq简化了识别最有价值变体的过程，以便进一步研究和应用。我们将DEQSeq应用于Cas12f-ABE和设计者重组酶的进化文库，识别具有改进特性的变体，以便将来应用。我们的研究结果表明，DEQSeq是加速酶发现和推进基因组编辑研究的强大工具。
