PDF(Pubmed)
Abstract：
Autoantibodies are the hallmark of autoimmunity, and specifically, antinuclear antibodies (ANA) are one of the most relevant antibodies present in systemic autoimmune diseases (AID). In the present study, we evaluate the relationship between ANA and sociodemographic and biobehavioral factors in a population with a low pre-test probability for systemic AID. ANA were determined in serum samples at baseline visit from 2997 participants from the Camargo Cohort using indirect immunofluorescence assay, and two solid phase assays (SPA), addressable laser bead immunoassay, and fluorescence enzyme immunoassay. Sociodemographic and biobehavioral features of the subjects were obtained at baseline visit using a structured questionnaire. The prevalence of ANA positive results was significantly higher when indirect immunofluorescence assay was used as screening method in comparison with SPAs, being higher in females, older subjects, and those with higher C-reactive protein levels. Considering biobehavioral features, the prevalence was higher in those individuals with a sedentary lifestyle, and in ex- and non-alcohol users. Moreover, considering the relevance of the antibody load using ANA Screen, the prevalence of the antibody load also increased with age, especially in females. In conclusion, the prevalence of ANA varies depending on sociodemographic and biobehavioral features of the subjects, which could be relevant specifically in a population with a low pre-test probability for systemic AIDs.
摘要：
自身抗体是自身免疫的标志,特别是,抗核抗体（ANA）是全身性自身免疫性疾病（AID）中存在的最相关的抗体之一。在本研究中,在系统性AID预测试概率较低的人群中,我们评估了ANA与社会人口统计学和生物行为因素之间的关系.使用间接免疫荧光测定法，在来自Camargo队列的2997名参与者的基线访视时测定血清样本中的ANA，和两个固相分析（SPA），可寻址激光珠免疫测定，和荧光酶免疫分析。使用结构化问卷在基线访问时获得受试者的社会人口统计学和生物行为特征。与SPAs相比，使用间接免疫荧光法作为筛查方法时，ANA阳性结果的患病率明显更高。女性更高，年长的科目，和那些C反应蛋白水平较高的人。考虑到生物行为特征，在那些久坐不动的人中患病率更高，以及前和非酒精使用者。此外，考虑使用ANA筛选的抗体负荷的相关性，抗体负荷的患病率也随着年龄的增长而增加，尤其是女性。总之,ANA的患病率取决于受试者的社会人口统计学和生物行为特征，这可能与系统性AID的预测试概率低的人群特别相关。
