Abstract：
BACKGROUND: This study determined the prevalence of aggressive (molar-incisor pattern) (Ag/MI) periodontitis and assessed the associated subgingival bacterial-herpesvirus microbiota in Pueblo Indian adolescents in the southwestern United States.
METHODS: The study included 240 Pueblo Indian adolescents, aged 13-20 years old, residing in three Rio Grande River villages in New Mexico and the Hopi Pueblo reservation in Arizona. Adolescents with Ag/MI periodontitis or periodontal health provided subgingival samples for culture of bacterial pathogens and for polymerase chain reaction detection of periodontal herpesviruses.
RESULTS: Ag/MI periodontitis was detected in 22 (9.2%) Pueblo Indian adolescents, with 21 exhibiting a localized molar-incisor breakdown pattern. Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, and other red/orange complex bacterial pathogens predominated in Ag/MI periodontitis, whereas periodontal health yielded mainly viridans streptococci and Actinomyces species. Periodontal herpesviruses demonstrated a 3.5 odds ratio relationship with Ag/MI periodontitis. The only adolescent with generalized Ag/MI periodontitis harbored viral co-infection by cytomegalovirus plus Epstein-Barr virus Type 1, in addition to A. actinomycetemcomitans, P. gingivalis, and several other periodontopathic bacteria.
CONCLUSIONS: Pueblo Indian adolescents showed an unusually high prevalence of early-age Ag/MI periodontitis predominated by periodontopathic bacteria and herpesviruses suspected to be major etiologic agents of the disease.
METHODS: The study included 240 Pueblo Indian adolescents, aged 13-20 years old, residing in three Rio Grande River villages in New Mexico and the Hopi Pueblo reservation in Arizona. Adolescents with Ag/MI periodontitis or periodontal health provided subgingival samples for culture of bacterial pathogens and for polymerase chain reaction detection of periodontal herpesviruses.
RESULTS: Ag/MI periodontitis was detected in 22 (9.2%) Pueblo Indian adolescents, with 21 exhibiting a localized molar-incisor breakdown pattern. Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, and other red/orange complex bacterial pathogens predominated in Ag/MI periodontitis, whereas periodontal health yielded mainly viridans streptococci and Actinomyces species. Periodontal herpesviruses demonstrated a 3.5 odds ratio relationship with Ag/MI periodontitis. The only adolescent with generalized Ag/MI periodontitis harbored viral co-infection by cytomegalovirus plus Epstein-Barr virus Type 1, in addition to A. actinomycetemcomitans, P. gingivalis, and several other periodontopathic bacteria.
CONCLUSIONS: Pueblo Indian adolescents showed an unusually high prevalence of early-age Ag/MI periodontitis predominated by periodontopathic bacteria and herpesviruses suspected to be major etiologic agents of the disease.
摘要：
背景：这项研究确定了美国西南部Pueblo印度青少年中侵袭性（磨牙切牙型）（Ag/MI）牙周炎的患病率，并评估了相关的龈下细菌-疱疹病毒微生物群。
方法：该研究包括240名印度普韦布洛青少年，13-20岁,居住在新墨西哥州的三个里奥格兰德河村庄和亚利桑那州的霍皮普韦布洛保留地。患有Ag/MI牙周炎或牙周健康的青少年提供了牙龈下样品，用于培养细菌病原体和聚合酶链反应检测牙周疱疹病毒。
结果：在22名（9.2％）印度普韦布洛青少年中检测到Ag/MI牙周炎，21例表现出局部的磨牙切牙分解模式。放线菌聚集杆菌，牙龈卟啉单胞菌,和其他红色/橙色复合细菌病原体在Ag/MI牙周炎中占主导地位，而牙周健康主要产生绿柱虫链球菌和放线菌。牙周炎疱疹病毒与Ag/MI牙周炎的比值比为3.5。除放线菌外，唯一患有广泛性Ag/MI牙周炎的青少年还患有巨细胞病毒加爱泼斯坦-巴尔病毒1型病毒的共同感染，牙龈卟啉单胞菌，和其他几种牙周病菌。
结论：普韦布洛印度青少年表现出异常高的早龄Ag/MI牙周炎患病率，以牙周病菌和疱疹病毒为主，怀疑是该病的主要病因。
方法：该研究包括240名印度普韦布洛青少年，13-20岁,居住在新墨西哥州的三个里奥格兰德河村庄和亚利桑那州的霍皮普韦布洛保留地。患有Ag/MI牙周炎或牙周健康的青少年提供了牙龈下样品，用于培养细菌病原体和聚合酶链反应检测牙周疱疹病毒。
结果：在22名（9.2％）印度普韦布洛青少年中检测到Ag/MI牙周炎，21例表现出局部的磨牙切牙分解模式。放线菌聚集杆菌，牙龈卟啉单胞菌,和其他红色/橙色复合细菌病原体在Ag/MI牙周炎中占主导地位，而牙周健康主要产生绿柱虫链球菌和放线菌。牙周炎疱疹病毒与Ag/MI牙周炎的比值比为3.5。除放线菌外，唯一患有广泛性Ag/MI牙周炎的青少年还患有巨细胞病毒加爱泼斯坦-巴尔病毒1型病毒的共同感染，牙龈卟啉单胞菌，和其他几种牙周病菌。
结论：普韦布洛印度青少年表现出异常高的早龄Ag/MI牙周炎患病率，以牙周病菌和疱疹病毒为主，怀疑是该病的主要病因。
