PDF(Pubmed)
Abstract：
OBJECTIVE: In this study, we aimed to evaluate the usability single nucleotide polymorphisms (SNPs) for parentage testing of horse breeds in Korea.
METHODS: The genotypes of 93 horse samples (38 Thoroughbred horses, 17 Jeju horses, 20 Quarter horses, and 18 American miniature horses) were determined using 15 microsatellite (Ms) markers (AHT4, AHT5, ASB2, ASB17, ASB23, CA425, HMS1, HMS2, HMS3, HMS6, HMS7, HTG4, HTG10, LEX3, and VHL20) and 101 SNP markers.
RESULTS: Paternity tests were performed using 15 Ms markers and 101 SNP markers in Thoroughbred horses and Quarter horses. AHT5, ASB2, ASB17, ASB23, CA425, HMS7, HTG10, and LEX3 did not follow Mendelian inheritance in Thoroughbred horses, whereas in Quarter horses, only AHT4, ASB2, and HMS2 showed Mendelian inheritance, consequently, paternity was not established. Meanwhile, 31 markers, including MNEc_2_2_ 2_98568918_BIEC2_502451, in Thoroughbred horses, and 30 markers, including MNEc_ 2_30_7430735_BIEC2_816793, in Quarter horses did not conform with Mendelian inheritance and therefore, could not be used for establishing parentage.
CONCLUSIONS: The possibility of replacing Ms markers with SNP markers for paternity testing in horses was confirmed. However, further research using more samples is necessary.
METHODS: The genotypes of 93 horse samples (38 Thoroughbred horses, 17 Jeju horses, 20 Quarter horses, and 18 American miniature horses) were determined using 15 microsatellite (Ms) markers (AHT4, AHT5, ASB2, ASB17, ASB23, CA425, HMS1, HMS2, HMS3, HMS6, HMS7, HTG4, HTG10, LEX3, and VHL20) and 101 SNP markers.
RESULTS: Paternity tests were performed using 15 Ms markers and 101 SNP markers in Thoroughbred horses and Quarter horses. AHT5, ASB2, ASB17, ASB23, CA425, HMS7, HTG10, and LEX3 did not follow Mendelian inheritance in Thoroughbred horses, whereas in Quarter horses, only AHT4, ASB2, and HMS2 showed Mendelian inheritance, consequently, paternity was not established. Meanwhile, 31 markers, including MNEc_2_2_ 2_98568918_BIEC2_502451, in Thoroughbred horses, and 30 markers, including MNEc_ 2_30_7430735_BIEC2_816793, in Quarter horses did not conform with Mendelian inheritance and therefore, could not be used for establishing parentage.
CONCLUSIONS: The possibility of replacing Ms markers with SNP markers for paternity testing in horses was confirmed. However, further research using more samples is necessary.
摘要：
目的：在本研究中，我们旨在评估韩国马品种亲子关系测试的可用性单核苷酸多态性(SNPs)。
方法：93匹马样本的基因型（38匹纯种马，17匹济州岛马，20匹四分之一的马，使用15个微卫星（Ms）标记（AHT4，AHT5，ASB2，ASB17，ASB23，CA425，HMS1，HMS2，HMS3，HMS6，HMS7，HTG4，HTG10，LEX3和VHL20）和101个SNP标记。
结果：使用纯种马和四分之一马的15个Ms标记和101个SNP标记进行亲子鉴定。AHT5，ASB2，ASB17，ASB23，CA425，HMS7，HTG10和LEX3在纯种马中没有遵循孟德尔遗传，而在四分之一马，只有AHT4、ASB2和HMS2表现出孟德尔遗传，因此,亲子关系尚未确定。同时,31个标记，包括MNEc_2_2_2_98568918_BIEC2_502451，在纯种马中，和30个标记，包括MNEC_2_30_7430735_BIEC2_816793，四分之一马匹不符合孟德尔遗传，因此，不能用于建立亲子关系。
结论：证实了用SNP标记代替Ms标记用于马匹亲子鉴定的可能性。然而,使用更多样本的进一步研究是必要的。
方法：93匹马样本的基因型（38匹纯种马，17匹济州岛马，20匹四分之一的马，使用15个微卫星（Ms）标记（AHT4，AHT5，ASB2，ASB17，ASB23，CA425，HMS1，HMS2，HMS3，HMS6，HMS7，HTG4，HTG10，LEX3和VHL20）和101个SNP标记。
结果：使用纯种马和四分之一马的15个Ms标记和101个SNP标记进行亲子鉴定。AHT5，ASB2，ASB17，ASB23，CA425，HMS7，HTG10和LEX3在纯种马中没有遵循孟德尔遗传，而在四分之一马，只有AHT4、ASB2和HMS2表现出孟德尔遗传，因此,亲子关系尚未确定。同时,31个标记，包括MNEc_2_2_2_98568918_BIEC2_502451，在纯种马中，和30个标记，包括MNEC_2_30_7430735_BIEC2_816793，四分之一马匹不符合孟德尔遗传，因此，不能用于建立亲子关系。
结论：证实了用SNP标记代替Ms标记用于马匹亲子鉴定的可能性。然而,使用更多样本的进一步研究是必要的。
