PDF(Pubmed)
Abstract：
Mounting evidence indicate that cuproptosis, a novel form of programmed cell death, contributes to cancer development and progression. However, a comprehensive analysis regarding the expressions, functions, and regulatory network of cuproptosis-related genes is still lacking. In the present work, cuproptosis-related genes, upstream miRNAs and lncRNAs, and clinical data of breast cancer from TCGA database were analyzed by R language including Cox regression analysis, correlation calculation, ROC curve construction, and survival evaluation, and were further verified by public-available databases. Chemosensitivity and immune infiltration were also evaluated by online tools. SLC31A1 was significantly increased in breast cancer samples than those in normal tissues. SLC31A1 was negatively related to a favorable outcome in breast cancer, and the AUC value increased with the prolongation of follow-up time. LINC01614 and miR-204-5p were potential upstream regulators of SLC31A1. Moreover, SLC31A1 was significantly positively correlated with different immune cells infiltration, immune cell biomarkers, and immune checkpoints in breast cancer. SLC31A1 was a potential cuproptosis-related gene in breast cancer, which was significantly upregulated and was able to predict diagnosis, prognosis, chemosensitivity, and immune infiltration. LINC01640/miR-204-5p/SLC31A1 might be a significant and promising axis during cuproptosis in breast cancer.
摘要：
越来越多的证据表明倾斜，一种新形式的程序性细胞死亡，有助于癌症的发展和进展。然而,对表达式的全面分析，功能,目前还缺乏与角化相关基因的调控网络。在目前的工作中,凋亡相关基因，上游miRNAs和lncRNAs，用R语言分析TCGA数据库中乳腺癌的临床数据,包括Cox回归分析,相关性计算，ROC曲线构建,和生存评估，并通过公共数据库进一步验证。还通过在线工具评估了化学敏感性和免疫浸润。SLC31A1在乳腺癌样本中明显高于正常组织。SLC31A1与乳腺癌的良好预后呈负相关，AUC值随着随访时间的延长而增加。LINC01614和miR-204-5p是SLC31A1的潜在上游调节因子。此外，SLC31A1与不同免疫细胞浸润呈显著正相关,免疫细胞生物标志物，和乳腺癌的免疫检查点。SLC31A1是一个潜在的乳腺癌细胞凋亡相关基因,显著上调并能够预测诊断，预后,化学敏感性，和免疫浸润。LINC01640/miR-204-5p/SLC31A1可能是乳腺癌角化过程中一个重要且有希望的轴。
