PDF(Pubmed)
Abstract：
Understanding the genomic underpinnings of thermal adaptation is a hot topic in eco-evolutionary studies of parasites. Marine heteroxenous parasites have complex life cycles encompassing a free-living larval stage, an ectothermic intermediate host and a homeothermic definitive host, thus representing compelling systems for the study of thermal adaptation. The Antarctic anisakid Contracaecum osculatum sp. D is a marine parasite able to survive and thrive both at very cold and warm temperatures within the environment and its hosts. Here, a de novo transcriptome of C. osculatum sp. D was generated for the first time, by performing RNA-Seq experiments on a set of individuals exposed to temperatures experienced by the nematode during its life cycle. The analysis generated 425,954,724 reads, which were assembled and then annotated. The high-quality assembly was validated, achieving over 88% mapping against the transcriptome. The transcriptome of this parasite will represent a valuable genomic resource for future studies aimed at disentangling the genomic architecture of thermal tolerance and metabolic pathways related to temperature stress.
摘要：
了解热适应的基因组基础是寄生虫生态进化研究的热门话题。海洋异氧寄生虫具有复杂的生命周期，包括自由生活的幼虫阶段，放热的中间宿主和稳态的最终宿主，因此代表了用于热适应研究的令人信服的系统。南极anisakid章鱼茎。D是一种海洋寄生虫，能够在环境及其宿主中非常寒冷和温暖的温度下生存和茁壮成长。这里,C.osculatumsp.的从头转录组。D是第一次产生的,通过对一组暴露于线虫生命周期中经历的温度的个体进行RNA-Seq实验。分析产生425,954,724个读段，它们被组装起来，然后被注释。高质量的装配得到了验证，实现了超过88%的转录组映射。该寄生虫的转录组将代表未来研究的宝贵基因组资源，旨在解开与温度胁迫相关的热耐受性和代谢途径的基因组结构。
