Abstract:
Sugarcane, a major sugar and energy crop worldwide faces an increasing demand for higher yields. Identifying yield-related markers and candidate genes is valuable for breeding high-yield varieties using molecular techniques. In this work, seven yield-related traits were evaluated in a diversity panel of 159 genotypes, derived from Tripidium arundinaceum, Saccharum spontaneum, and modern sugarcane genotypes. All traits exhibited significant genetic variance with high heritability and high correlations. Genetic diversity analysis reveals a genomic decay of 23 kb and an average single nucleotide polymorphism (SNP) number of 25,429 per genotype. These 159 genotypes were divided into 4 subgroups. Genome-wide association analysis identified 47 SNPs associated with brix, spanning 36 quantitative trait loci (QTLs), and 138 SNPs for other traits across 104 QTLs, covering all 32 chromosomes. Interestingly, 12 stable QTLs associated with yield-related traits were identified, which contained 35 candidate genes. This work provides markers and candidate genes for marker-assisted breeding to improve sugarcane yields.
摘要:
甘蔗,全球主要的糖和能源作物面临着越来越高的产量需求。识别与产量相关的标记和候选基因对于使用分子技术育种高产品种具有重要意义。在这项工作中,在159个基因型的多样性小组中评估了7个产量相关性状,来源于高云三嗪,糖精,和现代甘蔗基因型。所有性状均表现出显著的遗传变异,遗传力高,相关性高。遗传多样性分析显示基因组衰减为23kb,每个基因型的平均单核苷酸多态性(SNP)数为25,429。这159个基因型分为4个亚组。全基因组关联分析确定了47个与白利糖度相关的SNP,跨越36个数量性状基因座(QTL),以及104个QTL中其他性状的138个SNP,覆盖所有32条染色体.有趣的是,确定了12个与产量相关性状相关的稳定QTL,其中包含35个候选基因。这项工作为标记辅助育种提供了标记和候选基因,以提高甘蔗产量。