Abstract：
OBJECTIVE: Identification of DNT, a rare partial D, can be challenging, as it is difficult to distinguish from D+. This study aimed to identify DNT individuals by analyzing the DNT proband\'s family members, characterize DNT, and propose management strategies.
METHODS: Family members of the first Korean DNT proband were recruited. RHD genotyping was conducted, and weak D tests were carried out using several anti-D reagents.
RESULTS: Three DNT individuals were identified among 6 family members, including 1 with an anti-D alloantibody. As DNT red cells exhibited strong reactivity with all anti-D clones, DNT was serologically indistinguishable from D+. Moreover, unusual serologic findings in DNT individuals only became apparent after anti-D alloimmunization.
CONCLUSIONS: We recommend DNT individuals as candidates for Rh immune globulin prophylaxis during the perinatal period and transfusions with D- blood components. An anticipatory RHD genotyping is suggested for partial D family members to prevent potential partial D individuals from becoming alloimmunized.
METHODS: Family members of the first Korean DNT proband were recruited. RHD genotyping was conducted, and weak D tests were carried out using several anti-D reagents.
RESULTS: Three DNT individuals were identified among 6 family members, including 1 with an anti-D alloantibody. As DNT red cells exhibited strong reactivity with all anti-D clones, DNT was serologically indistinguishable from D+. Moreover, unusual serologic findings in DNT individuals only became apparent after anti-D alloimmunization.
CONCLUSIONS: We recommend DNT individuals as candidates for Rh immune globulin prophylaxis during the perinatal period and transfusions with D- blood components. An anticipatory RHD genotyping is suggested for partial D family members to prevent potential partial D individuals from becoming alloimmunized.
摘要：
目的：鉴定DNT，罕见的局部D,可能是具有挑战性的，因为很难区分D+。本研究旨在通过分析DNT先证者的家庭成员来识别DNT个体，表征DNT，并提出管理策略。
方法：招募第一个韩国DNT先证者的家庭成员。进行了RHD基因分型，使用几种抗D试剂进行弱D测试。
结果：在6个家庭成员中确定了3个DNT个体，包括1种抗D同种抗体。由于DNT红细胞对所有抗D克隆都表现出强烈的反应性，DNT在血清学上与D+没有区别。此外，DNT个体的异常血清学发现仅在抗D同种免疫后才变得明显。
结论：我们推荐DNT个体作为围产期Rh免疫球蛋白预防和D-血成分输血的候选者。建议对部分D家族成员进行预期的RHD基因分型，以防止潜在的部分D个体被同种免疫。
方法：招募第一个韩国DNT先证者的家庭成员。进行了RHD基因分型，使用几种抗D试剂进行弱D测试。
结果：在6个家庭成员中确定了3个DNT个体，包括1种抗D同种抗体。由于DNT红细胞对所有抗D克隆都表现出强烈的反应性，DNT在血清学上与D+没有区别。此外，DNT个体的异常血清学发现仅在抗D同种免疫后才变得明显。
结论：我们推荐DNT个体作为围产期Rh免疫球蛋白预防和D-血成分输血的候选者。建议对部分D家族成员进行预期的RHD基因分型，以防止潜在的部分D个体被同种免疫。
