Abstract：
In recent years, the CRISPR/Cas9 system has become a rapidly advancing gene editing technology with significant advantages in various fields, particularly biomedicine. Liver cancer is a severe malignancy that threatens human health and is primarily treated with surgery, radiotherapy, and chemotherapy. However, surgery may not be suitable for advanced cases of liver cancer with distant metastases. Moreover, radiotherapy and chemotherapy have low specificity and numerous side effects that limit their effectiveness; therefore, more effective and safer treatments are required. With the advancement of the biomolecular mechanism of cancer, CRISPR/Cas9 gene editing technology has been widely used in the study of liver cancer to gain insights into gene functions, establish tumor models, screen tumor phenotype-related genes, and perform gene therapy. This review outlines the research progress of CRISPR/Cas9 gene editing technology in the treatment of liver cancer and provides a relevant theoretical basis for its research and application in the treatment of liver cancer.
摘要：
近年来,CRISPR/Cas9系统已经成为一种快速发展的基因编辑技术，在各个领域都具有显著的优势，尤其是生物医学。肝癌是威胁人类健康的严重恶性肿瘤，主要通过手术治疗，放射治疗,和化疗。然而,手术可能不适合晚期肝癌远处转移的病例。此外，放疗和化疗特异性低，副作用众多，限制了其有效性；因此，需要更有效和更安全的治疗方法。随着肿瘤生物分子机制的发展,CRISPR/Cas9基因编辑技术已广泛用于肝癌的研究，以了解基因功能，建立肿瘤模型，筛选肿瘤表型相关基因，进行基因治疗.本文综述了CRISPR/Cas9基因编辑技术在肝癌治疗中的研究进展,为其在肝癌治疗中的研究与应用提供相关理论依据。
