Abstract：
OBJECTIVE: To study the impact of mycoplasma genitalium (Mg) infection on sperm DNA fragmentation in male infertility patients and its clinical significance.
METHODS: We performed flow cytometry and routine semen examination for 100 male infertility patients with Mg infection (the case group) and 200 infertile men without Mg infection (the control group). We treated the patients with the antibacterial drug Azithromycin and examined their sperm DNA fragmentation index (DFI) by flow cytometry after treatment.
RESULTS: The baseline sperm DFI was dramatically higher in the case group than in the normal controls (［40.1 ± 9.9］% vs ［5.4 ± 4.9］%, (P < 0.01), but reduced to (19.0 ± 9.0)% after antibacterial medication (P < 0.01). The count of immotile sperm was significantly decreased in the infertility patients after treatment (P < 0.05), but no statistically significant difference was observed in sperm concentration and motility before and after treatment (P > 0.05).
CONCLUSIONS: The sperm DFI is significantly higher in male infertility patients with Mg infection than in normal men, and antibacterial therapy can effectively reduce the level of Mg infection-induced sperm DNA fragmentation. The analysis of sperm DNA fragmentation plays a more significant role than routine semen examination in the diagnosis and treatment of male infertility with Mg infection.
METHODS: We performed flow cytometry and routine semen examination for 100 male infertility patients with Mg infection (the case group) and 200 infertile men without Mg infection (the control group). We treated the patients with the antibacterial drug Azithromycin and examined their sperm DNA fragmentation index (DFI) by flow cytometry after treatment.
RESULTS: The baseline sperm DFI was dramatically higher in the case group than in the normal controls (［40.1 ± 9.9］% vs ［5.4 ± 4.9］%, (P < 0.01), but reduced to (19.0 ± 9.0)% after antibacterial medication (P < 0.01). The count of immotile sperm was significantly decreased in the infertility patients after treatment (P < 0.05), but no statistically significant difference was observed in sperm concentration and motility before and after treatment (P > 0.05).
CONCLUSIONS: The sperm DFI is significantly higher in male infertility patients with Mg infection than in normal men, and antibacterial therapy can effectively reduce the level of Mg infection-induced sperm DNA fragmentation. The analysis of sperm DNA fragmentation plays a more significant role than routine semen examination in the diagnosis and treatment of male infertility with Mg infection.
摘要：
目的：研究生殖支原体(Mg)感染对男性不育患者精子DNA断裂的影响及其临床意义。
方法：我们对100例有Mg感染的男性不育患者（病例组）和200例无Mg感染的男性不育患者（对照组）进行了流式细胞术和常规精液检查。我们使用抗菌药物阿奇霉素对患者进行治疗，并在治疗后通过流式细胞术检查其精子DNA碎片指数（DFI）。
结果：病例组的基线精子DFI明显高于正常对照组（［40.1±9.9］%vs［5.4±4.9］%，(P<0.01),但抗菌药物治疗后降低至(19.0±9.0)%(P<0.01)。治疗后不孕症患者的精子数量明显下降(P<0.05),但治疗前后精子浓度和活力差异无统计学意义(P>0.05)。
结论：镁感染男性不育患者精子DFI明显高于正常男性，抗菌治疗能有效降低Mg感染诱导的精子DNA断裂水平。精子DNA碎片分析在Mg感染男性不育症的诊断和治疗中，比常规精液检查具有更重要的作用。
方法：我们对100例有Mg感染的男性不育患者（病例组）和200例无Mg感染的男性不育患者（对照组）进行了流式细胞术和常规精液检查。我们使用抗菌药物阿奇霉素对患者进行治疗，并在治疗后通过流式细胞术检查其精子DNA碎片指数（DFI）。
结果：病例组的基线精子DFI明显高于正常对照组（［40.1±9.9］%vs［5.4±4.9］%，(P<0.01),但抗菌药物治疗后降低至(19.0±9.0)%(P<0.01)。治疗后不孕症患者的精子数量明显下降(P<0.05),但治疗前后精子浓度和活力差异无统计学意义(P>0.05)。
结论：镁感染男性不育患者精子DFI明显高于正常男性，抗菌治疗能有效降低Mg感染诱导的精子DNA断裂水平。精子DNA碎片分析在Mg感染男性不育症的诊断和治疗中，比常规精液检查具有更重要的作用。
