PDF(Pubmed)
Abstract：
During early telencephalic development, intricate processes of regional patterning and neural stem cell (NSC) fate specification take place. However, our understanding of these processes in primates, including both conserved and species-specific features, remains limited. Here, we profiled 761,529 single-cell transcriptomes from multiple regions of the prenatal macaque telencephalon. We deciphered the molecular programs of the early organizing centers and their cross-talk with NSCs, revealing primate-biased galanin-like peptide (GALP) signaling in the anteroventral telencephalon. Regional transcriptomic variations were observed along the frontotemporal axis during early stages of neocortical NSC progression and in neurons and astrocytes. Additionally, we found that genes associated with neuropsychiatric disorders and brain cancer risk might play critical roles in the early telencephalic organizers and during NSC progression.
摘要：
在早期端脑发育过程中，区域模式和神经干细胞（NSC）命运规范的复杂过程发生。然而,我们对灵长类动物这些过程的理解，包括保守性和物种特异性特征，仍然有限。这里,我们对来自产前猕猴端脑多个区域的761,529个单细胞转录组进行了分析。我们破译了早期组织中心的分子程序及其与NSC的交叉对话，揭示了前腹侧端脑中灵长类动物偏向的甘丙肽样肽（GALP）信号。在新皮质NSC进展的早期阶段以及神经元和星形胶质细胞中，沿额颞轴观察到区域转录组变化。此外,我们发现,与神经精神疾病和脑癌风险相关的基因可能在早期端脑组织者和NSC进展过程中起关键作用.
