Abstract：
Herein, we introduced ortho-phthalaldehyde (OPA) for blocking free amino groups and established a simple and robust method for comprehensive profiling of protein terminome based on strong cation exchange chromatography (SCX) fractionation. With the highly efficient and chemoseletive amine-group blocking, we identified 2271 canonical human protein N-termini, 1650 canonical human protein C-termini, as well as 645 protein neo-N-termini from HeLa cells.
摘要：
在这里，我们引入邻苯二甲醛（OPA）用于阻断游离氨基，并基于强阳离子交换色谱（SCX）分级分离，建立了一种简单而可靠的方法来全面分析蛋白质末端。具有高效和化学选择性的胺基阻断作用，我们确定了2271个典型的人类蛋白质N端，1650规范的人类蛋白质C末端，以及来自HeLa细胞的645种蛋白质新N末端。
