{Reference Type}: Journal Article
{Title}: Highly efficient and chemoselective blocking of free amino group by ortho-phthalaldehyde (OPA) for comprehensive analysis of protein terminome.
{Author}: Wang T;Wang Z;Wang R;Zhang L;Zhang Y;Lu H;
{Journal}: Talanta
{Volume}: 267
{Issue}: 0
{Year}: 2024 Jan 15
{Factor}: 6.556
{DOI}: 10.1016/j.talanta.2023.125262
{Abstract}: Herein, we introduced ortho-phthalaldehyde (OPA) for blocking free amino groups and established a simple and robust method for comprehensive profiling of protein terminome based on strong cation exchange chromatography (SCX) fractionation. With the highly efficient and chemoseletive amine-group blocking, we identified 2271 canonical human protein N-termini, 1650 canonical human protein C-termini, as well as 645 protein neo-N-termini from HeLa cells.