%0 Journal Article
%T Highly efficient and chemoselective blocking of free amino group by ortho-phthalaldehyde (OPA) for comprehensive analysis of protein terminome.
%A Wang T
%A Wang Z
%A Wang R
%A Zhang L
%A Zhang Y
%A Lu H
%J Talanta
%V 267
%N 0
%D 2024 Jan 15
%M 37804787
%F 6.556
%R 10.1016/j.talanta.2023.125262
%X Herein, we introduced ortho-phthalaldehyde (OPA) for blocking free amino groups and established a simple and robust method for comprehensive profiling of protein terminome based on strong cation exchange chromatography (SCX) fractionation. With the highly efficient and chemoseletive amine-group blocking, we identified 2271 canonical human protein N-termini, 1650 canonical human protein C-termini, as well as 645 protein neo-N-termini from HeLa cells.