RESULTS: B. piscis OK324045 characterized by 16S rRNA is a potent EPS-forming organism and yielded a 6.42-fold increase upon supplementation of molasses (5%), MgSO4 (0.05%), and inoculum size (4%). The novel exopolysaccharide produced by Bacillus sp. (EPS-BP5M) was confirmed by the structural analysis. The findings indicated that the MFC\'s maximum close circuit voltage (CCV) was 265 mV. The strain enhanced the performance of DCMFC achieving maximum power density (PD) of 31.98 mW m-2, COD removal rate of 90.91%, and color removal of 27.68%. Furthermore, cyclic voltammetry (CV) revealed that anodic biofilms may directly transfer electrons to anodes without the use of external redox mediators. Additionally, CV measurements made at various sweep scan rates to evaluate the kinetic studies showed that the electron charge transfer was irreversible. The SEM images showed the biofilm growth distributed over the electrode\'s surface.
CONCLUSIONS: This study offers a novel B. piscis strain for EPS-BP5M production, COD removal, decolorization, and electricity generation of the optimized molasses medium in MFCs. The biosynthesis of EPS-BP5M by a Bacillus piscis strain and its electrochemical activity has never been documented before. The approach adopted will provide significant benefits to sugar industries by generating bioelectricity using molasses as fuel and providing a viable way to improve molasses wastewater treatment.
结果:B.以16SrRNA为特征的piscisOK324045是一种有效的EPS形成生物,在补充糖蜜(5%)后增加了6.42倍,MgSO4(0.05%),和接种物大小(4%)。由芽孢杆菌属产生的新型胞外多糖。通过结构分析证实了(EPS-BP5M)。结果表明,MFC的最大闭路电压(CCV)为265mV。该菌株提高了DCMFC的性能,最大功率密度(PD)为31.98mWm-2,COD去除率为90.91%,颜色去除率为27.68%。此外,循环伏安法(CV)表明,阳极生物膜可以直接将电子转移到阳极,而无需使用外部氧化还原介体。此外,在各种扫描扫描速率下进行的CV测量以评估动力学研究表明,电子电荷转移是不可逆的。SEM图像显示生物膜生长分布在电极表面。
结论:本研究为EPS-BP5M生产提供了一种新的双歧杆菌菌株,COD去除,脱色,优化糖蜜培养基在MFC中的发电。由比西芽孢杆菌菌株生物合成EPS-BP5M及其电化学活性以前从未被记录过。采用的方法将通过使用糖蜜作为燃料产生生物电并提供改善糖蜜废水处理的可行方法,从而为制糖工业带来重大利益。