Abstract：
Lifestyle factors such as sedentary behavior and consumption of certain medications can disturb the function of the male reproductive system. In the present study, we investigated the potential co-treatment effects of low-volume high-intensity interval training (HIIT) on markers of reproductive function in adult male Wistar rats under aspirin (ASA) treatment. Eighteen adult male Wistar rats were randomized into three groups: control (C), aspirin treatment (AT), and aspirin treatment + exercise (ATE). Animals in the AT and ATE groups received an oral subchronic dose of aspirin (12.5 mg/kg body mass). The exercise was performed three times per week for 6 weeks (4-6 reps of 10-s sprints). Serum testosterone level, sperm parameters (sperm count, viability, maturity, and DNA fragmentation), histomorphometric (Leydig cell, tubule diameter, thickness of tubular epithelium, and indices of spermatogenesis and spermiogenesis), and histochemical parameter (testicular fat density) were assessed. Results revealed that compared to the C group, ASA consumption led to a negative alteration in serum testosterone levels, sperm parameters, and histomorphometric and histochemical parameters (P < 0.05). However, there were no significant differences between the C and ATE groups in terms of serum testosterone level, number of Leydig cells, epididymal fat density, tubule diameter, epithelium height, immature-to-mature sperm ratio, and DNA breakage (P > 0.05). These findings suggest that ASA treatment is associated with deleterious changes in male reproductive parameters. However, low-volume HIIT may prevent ASA-induced male reproductive impairments and could be considered a potential prophylactic measure in subjects under ASA treatment.
摘要：
生活方式因素，如久坐的行为和某些药物的消费会扰乱男性生殖系统的功能。在本研究中,我们研究了小量高强度间歇训练(HIIT)对阿司匹林(ASA)治疗的成年雄性Wistar大鼠生殖功能标志物的潜在协同治疗作用.将18只成年雄性Wistar大鼠随机分为三组：对照组（C），阿司匹林治疗（AT），和阿司匹林治疗+运动（ATE）。AT和ATE组中的动物接受口服亚慢性剂量的阿司匹林(12.5mg/kg体重)。该运动每周进行3次，持续6周（4-6次10秒冲刺）。血清睾酮水平，精子参数（精子计数，生存能力，成熟，和DNA片段化)，组织形态学(Leydig细胞，小管直径，管状上皮的厚度，和精子发生和精子发生指数)，和组织化学参数（睾丸脂肪密度）进行评估。结果显示，与C组相比，ASA消耗导致血清睾酮水平的阴性变化，精子参数,和组织形态计量学和组织化学参数(P<0.05)。然而,在血清睾酮水平方面，C组和ATE组之间没有显着差异，Leydig细胞的数量,附睾脂肪密度,小管直径，上皮高度,未成熟与成熟的精子比例，和DNA断裂(P>0.05)。这些发现表明，ASA治疗与男性生殖参数的有害变化有关。然而,低容量HIIT可预防ASA诱导的男性生殖损伤,可被视为ASA治疗受试者的潜在预防措施.
