UNASSIGNED: This study aims to elucidate the archaeome structure and function in the porcine intestinal tract of healthy and H1N1 infected swine. We performed multi-omics analysis consisting of 16S rRNA gene profiling, metatranscriptomics and metaproteomics.
UNASSIGNED: We observed a significant increase from 0.48 to 4.50% of archaea in the intestinal tract microbiome along the ileum and colon, dominated by genera Methanobrevibacter and Methanosphaera. Furthermore, in feces of naïve and H1N1 infected swine, we observed significant but minor differences in the occurrence of archaeal phylotypes over the course of an infection experiment. Metatranscriptomic analysis of archaeal mRNAs revealed the major methanogenesis pathways of Methanobrevibacter and Methanosphaera to be hydrogenotrophic and methyl-reducing, respectively. Metaproteomics of archaeal peptides indicated some effects of the H1N1 infection on central metabolism of the gut archaea.
UNASSIGNED: Finally, this study provides the first multi-omics analysis and high-resolution insights into the structure and function of the porcine intestinal tract archaeome during a non-lethal Influenza A virus infection of the respiratory tract, demonstrating significant alterations in archaeal community composition and central metabolic functions.
■本研究旨在阐明健康和H1N1感染猪的猪肠道中的古细菌结构和功能。我们进行了由16SrRNA基因谱分析组成的多组学分析,元转录组学和元蛋白质组学。
■我们观察到沿回肠和结肠的肠道微生物组中古细菌从0.48到4.50%的显着增加,以甲烷杆菌属和甲烷属为主。此外,在幼稚和H1N1感染猪的粪便中,在感染实验过程中,我们观察到古细菌表型的发生有显着但很小的差异。古细菌mRNAs的代谢组学分析揭示了甲烷的主要产甲烷途径是氢营养和甲基还原,分别。古细菌肽的元蛋白质组学表明,H1N1感染对肠道古细菌的中枢代谢有一定影响。
■最后,这项研究为非致死性甲型流感病毒呼吸道感染期间猪肠道古细菌的结构和功能提供了第一个多组学分析和高分辨率见解,证明了古细菌群落组成和中心代谢功能的显着变化。