PDF(Pubmed)
Abstract：
Visual perception plays a crucial role in foraging, avoiding predators, mate selection, and communication. The regulation of color vision is largely dependent on opsin, which is the first step in the formation of the visual transduction cascade in photoreceptor cells. Short-wave-sensitive 1 (sws1) is a visual pigment that mediates short-wavelength light transduction in vertebrates. The depletion of sws1 resulted in increased M-opsin in mice. However, there is still no report on the visual function of sws1 in teleost fish. Here, we constructed the sws1 knockout medaka using CRISPR/Cas9 technology. The 6 dph (days post-hatching) medaka sws1-/- larvae exhibited significantly decreased food intake and total length at the first feeding stage, and the mRNA levels of orexigenic genes (npy and agrp) were significantly upregulated after feeding. The swimming speed was significantly reduced during the period of dark-light transition stimulation in the sws1-mutant larvae. Histological analysis showed that the thickness of the lens was reduced, whereas the thickness of the ganglion cell layer (GCL) was significantly increased in sws1-/- medaka larvae. Additionally, the deletion of sws1 decreased the mRNA levels of genes involved in phototransduction (gnb3b, grk7a, grk7b, and pde6c). We also observed increased retinal cell apoptosis and oxidative stress in sws1 knockout medaka larvae. Collectively, these results suggest that sws1 deficiency in medaka larvae may impair visual function and cause retinal cell apoptosis, which is associated with the downregulation of photoconduction expression and oxidative stress.
摘要：
视觉感知在觅食中起着至关重要的作用，避免捕食者,择偶,和沟通。色觉的调节在很大程度上依赖于视蛋白，这是感光细胞视觉转导级联形成的第一步。短波敏感1（sws1）是介导脊椎动物短波光转导的视觉色素。sws1的消耗导致小鼠中M-视蛋白增加。然而,目前还没有关于硬骨鱼sws1视觉功能的报告。这里,我们使用CRISPR/Cas9技术构建了sws1敲除medaka。6dph（孵化后的天数）medakasws1-/-幼虫在第一个摄食阶段表现出显着降低的食物摄入量和总长度，饲喂后，食欲基因（npy和agrp）的mRNA水平显着上调。在sws1突变幼虫的暗光过渡刺激期间，游泳速度显着降低。组织学分析显示晶状体厚度减小,而sw-/-medaka幼虫的神经节细胞层（GCL）的厚度显着增加。此外,sws1的缺失降低了参与光转导的基因的mRNA水平(gnb3b，grk7a,grk7b,和PDE6c)。我们还观察到sws1敲除的medaka幼虫的视网膜细胞凋亡和氧化应激增加。总的来说，这些结果表明，麻黄幼虫的sws1缺乏可能损害视觉功能并导致视网膜细胞凋亡，这与光导表达和氧化应激的下调有关。
