最近,纳米二氧化钛(nano-TiO2)已广泛分布在地表水中。然而,关于它对鱼类中枢神经系统的影响的报道很少。在这项研究中,我们研究了纳米TiO2在暴露后是否会进入medaka大脑及其对大脑的影响。在暴露于0.01g/L纳米TiO23、10和20d后,检查了海洋medaka的大脑。在处理过的鱼的端脑中发现了纳米TiO2样颗粒。无明显的脑组织病理损伤。无cr的不规则线粒体数量增加。凋亡相关基因的基因表达,casp8,bcl2b,还有bax,纳米TiO2组在3d时显著降低。相比之下,与焦亡相关的基因,gsdmeb和casp1,以及炎症相关因子,il18,显著增加。作为激活的小胶质细胞标记,cd68的mRNA表达在纳米TiO2处理组中显著增加。此外,CD68蛋白表达在10d时也显著增加。我们表明,纳米TiO2可以改变medaka端脑的线粒体形态,导致小胶质细胞活化和焦亡。
Recently, nano-titanium dioxide (nano-TiO2) has been widely distributed over surface water. However, there are few reports on its effects on the central nervous system of fish. In this study, we investigated whether nano-TiO2 enters the medaka brain after exposure and its effect on the brain. Marine medaka brains were examined after exposure to 0.01 g/L nano-TiO2 for 3, 10, and 20 d. Nano-TiO2-like particles were found in the telencephalon of treated fish. There was no obvious brain histopathological injury. The number of irregular mitochondria with absent cristae increased. Gene expression of the apoptosis-related genes, casp8, bcl2b, and bax, decreased significantly in the nano-TiO2 group at 3 d. In contrast, the pyroptosis-related genes, gsdmeb and casp1, and inflammation-related factor, il18, increased significantly. As an activated microglia marker, mRNA expression of cd68 increased significantly in the nano-TiO2 treated group. Moreover, CD68 protein expression also increased significantly at 10 d. Altogether, we show that nano-TiO2 can alter mitochondrial morphology in the telencephalon of medaka, leading to microglial activation and pyroptosis.