Abstract：
The potential of using commercial peroxyacetic acid (PAA) for Vibrio parahaemolyticus sanitization was evaluated. Commercial PAA of 0.005 % (v/v, PAA: 2.24 mg/L, hydrogen peroxide: 11.79 mg/L) resulted in a planktonic cell reduction of >7.00 log10 CFU/mL when initial V. parahaemolyticus cells averaged 7.64 log10 CFU/mL. For cells on stainless steel coupons, treatment of 0.02 % PAA (v/v, PAA: 8.96 mg/L, hydrogen peroxide: 47.16 mg/L) achieved >5.00 log10 CFU/cm2 reductions in biofilm cells for eight strains but not for the two strongest biofilm formers. PAA of 0.05 % (v/v, PAA: 22.39 mg/L, hydrogen peroxide: 117.91 mg/L) was required to inactivate >5.00 log10 CFU/cm2 biofilm cells from mussel shell surfaces. The detection of PAA residues after biofilm treatment demonstrated that higher biofilm production resulted in higher PAA residues (p < 0.05), suggesting biofilm is acting as a barrier interfering with PAA diffusing into the matrices. Based on the comparative analysis of genomes, robust biofilm formation and metabolic heterogeneity within niches might have contributed to the variations in PAA resistance of V. parahaemolyticus biofilms.
摘要：
评估了使用商业过氧乙酸（PAA）进行副溶血性弧菌消毒的潜力。商业PAA为0.005%(v/v，PAA:2.24mg/L,过氧化氢：11.79mg/L）导致当初始副溶血性弧菌细胞平均7.64log10CFU/mL时，浮游细胞减少>7.00log10CFU/mL。对于不锈钢试样上的细胞，0.02%PAA（v/v，PAA:8.96mg/L,过氧化氢：47.16mg/L）在8个菌株的生物膜细胞中实现了>5.00log10CFU/cm2的减少，但对于两个最强的生物膜形成剂却没有。PAA为0.05%(v/v,PAA:22.39mg/L,过氧化氢：117.91mg/L）需要灭活来自贻贝壳表面的>5.00log10CFU/cm2生物膜细胞。生物膜处理后PAA残留的检测表明，较高的生物膜产量导致较高的PAA残留（p<0.05），这表明生物膜是干扰PAA扩散到基质中的屏障。基于基因组的比较分析,强烈的生物膜形成和生态位内的代谢异质性可能导致副溶血性弧菌生物膜对PAA抗性的变化。
