Abstract：
Porphyromonas gingivalis is the etiological agent of chronic periodontitis. Menadione (vitamin K3) and phylloquinone (vitamin K1) are well-known growth factors for P. gingivalis, while menadione is widely used in growth experiments. Here we attempted to determine the differences in phylloquinone and menadione in P. gingivalis growth experiments, which have not been well studied to date.
We investigated the effects of menadione and phylloquinone on the growth of two W83 strains and seven ATCC 33277 strains of P. gingivalis.
The ATCC 33277 strains grew well with phylloquinone at 2.9 μM in a complex medium (nutrient medium) and at 29 μM in two minimal media. In contrast, the W83 strains grew well without menadione or phylloquinone in three different culture media. Menadione at 2.9 μM, the conventionally used concentration for culturing P. gingivalis, supported the growth of most ATCC 33277 strains but inhibited the growth of some W83 and ATCC 33277 strains. Furthermore, menadione at 14.5 μM frequently inhibited cell growth, while phylloquinone at 145 μM promoted cell growth.
These results indicate that menadione and phylloquinone act as growth factors for ATCC 33277 but that menadione also can inhibit P. gingivalis growth. Thus, we propose that phylloquinone be used instead of menadione in P. gingivalis growth experiments requiring vitamin K.
We investigated the effects of menadione and phylloquinone on the growth of two W83 strains and seven ATCC 33277 strains of P. gingivalis.
The ATCC 33277 strains grew well with phylloquinone at 2.9 μM in a complex medium (nutrient medium) and at 29 μM in two minimal media. In contrast, the W83 strains grew well without menadione or phylloquinone in three different culture media. Menadione at 2.9 μM, the conventionally used concentration for culturing P. gingivalis, supported the growth of most ATCC 33277 strains but inhibited the growth of some W83 and ATCC 33277 strains. Furthermore, menadione at 14.5 μM frequently inhibited cell growth, while phylloquinone at 145 μM promoted cell growth.
These results indicate that menadione and phylloquinone act as growth factors for ATCC 33277 but that menadione also can inhibit P. gingivalis growth. Thus, we propose that phylloquinone be used instead of menadione in P. gingivalis growth experiments requiring vitamin K.
摘要：
目的：牙龈卟啉单胞菌是慢性牙周炎的病因。甲萘醌（维生素K3）和苯醌（维生素K1）是众所周知的牙龈卟啉单胞菌的生长因子，而甲萘醌被广泛用于生长实验。在这里，我们试图确定在牙龈卟啉单胞菌生长实验中苯醌和甲萘醌的差异，到目前为止还没有得到很好的研究。
方法：我们研究了甲萘醌和页醌对牙龈卟啉单胞菌的两个W83菌株和七个ATCC33277菌株生长的影响。
结果:ATCC33277菌株在复合培养基(营养培养基)中在2.9μM的页醌和在两种基本培养基中在29μM的页醌下生长良好。相比之下,在三种不同的培养基中，W83菌株在没有甲萘醌或叶醌的情况下生长良好。2.9μM的甲萘醌，通常用于培养牙龈卟啉单胞菌的浓度，支持大多数ATCC33277菌株的生长，但抑制某些W83和ATCC33277菌株的生长。此外，甲萘醌在14.5μM经常抑制细胞生长，而145μM的苯醌促进细胞生长。
结论：这些结果表明，甲萘醌和页醌作为ATCC33277的生长因子，但甲萘醌也可以抑制牙龈卟啉单胞菌的生长。因此，我们建议在需要维生素K的牙龈卟啉单胞菌生长实验中使用苯醌代替甲萘醌。
方法：我们研究了甲萘醌和页醌对牙龈卟啉单胞菌的两个W83菌株和七个ATCC33277菌株生长的影响。
结果:ATCC33277菌株在复合培养基(营养培养基)中在2.9μM的页醌和在两种基本培养基中在29μM的页醌下生长良好。相比之下,在三种不同的培养基中，W83菌株在没有甲萘醌或叶醌的情况下生长良好。2.9μM的甲萘醌，通常用于培养牙龈卟啉单胞菌的浓度，支持大多数ATCC33277菌株的生长，但抑制某些W83和ATCC33277菌株的生长。此外，甲萘醌在14.5μM经常抑制细胞生长，而145μM的苯醌促进细胞生长。
结论：这些结果表明，甲萘醌和页醌作为ATCC33277的生长因子，但甲萘醌也可以抑制牙龈卟啉单胞菌的生长。因此，我们建议在需要维生素K的牙龈卟啉单胞菌生长实验中使用苯醌代替甲萘醌。
