■在nu/nu雄性小鼠中植入的雄激素非依赖性人DU145前列腺转移癌的异种移植物显示,在由甲萘醌亚硫酸氢盐和抗坏血酸钠(VK3:VC)的组合组成的促氧化剂抗癌治疗后,具有显着的存活率。
■移植的隔膜癌样本来自存活时间最长的小鼠,腹膜内(IP),或口服和IP治疗组均采用光照评估,扫描,和透射电子显微镜来分析形态损伤。
■与先前未经处理的体外癌的精细结构数据相比,口腔引起的变化,IP,并口服IPVK3:VC治疗拆除了那些自体移植的异种移植物,坏死萎缩是通过细胞的氧化应激完成的,其损伤是由重新激活的脱氧核糖核酸酶和核糖核酸酶引起的。肿瘤破坏是由细胞核成分的不可逆损伤引起的,内质网,还有线粒体.其他改变包括细胞骨架的改变,这些改变导致了称为“自体分裂”的特征性自我切除。“所有这些损伤导致弹性癌细胞坏死细胞死亡。
■在人DU145前列腺异种移植物中由VK3:VC促氧化剂组合引起的精细结构损伤通过组织化学和生物分子研究证实了在体外和其他细胞系中显示的那些。这些破坏不会对正常组织造成损害;因此,我们的数据为上述联合治疗前列腺癌和其他癌症提供了支持.
UNASSIGNED: Xenografts of androgen-independent human DU145 prostate metastatic carcinomas implanted in nu/nu male mice have revealed a significant survival after a prooxidant anticancer treatment consisting of a combination of menadione bisulfite and sodium ascorbate (VK3:VC).
UNASSIGNED: Implanted samples of diaphragm carcinomas from longest survived mice from either oral, intraperitoneal (IP), or both oral and IP treatment groups were assessed with light, scanning, and transmission electron microscopy to analyze morphologic damages.
UNASSIGNED: Compared with previous fine structure data of in vitro untreated carcinomas, the changes induced by oral, IP, and oral with IP VK3:VC treatment dismantled those xenografts with autoschizis, and necrotic atrophy was accomplished by cell\'s oxidative stress whose injuries were consequent to reactivated deoxyribonucleases and ribonucleases. Tumor destructions resulted from irreversible damages of nucleus components, endoplasmic reticulum, and mitochondria there. Other alterations included those of the cytoskeleton that resulted in characteristic self-excisions named \" autoschizis.\" All these injuries lead resilient cancer cells to necrotic cell death.
UNASSIGNED: The fine structure damages caused by VK3:VC prooxidant combination in the human DU145 prostate xenografts confirmed those shown in vitro and of other cell lines with histochemistry and biomolecular investigations. These devastations incurred without damage to normal tissues; thus, our data brought support for the above combination to assist in the treatment of prostate cancers and other cancers.