PDF(Pubmed)
Abstract：
Nuclear pore complexes are large multicomponent protein complexes that are embedded in the nuclear envelope, where they mediate nucleocytoplasmic transport. In addition to supporting transport, nuclear pore components, termed nucleoporins (Nups), can interact with chromatin and influence genome function. A subset of Nups can also localize to the nuclear interior and bind chromatin intranuclearly, providing an opportunity to investigate chromatin-associated functions of Nups outside of the transport context. This review focuses on the gene regulatory functions of such intranuclear Nups, with a particular emphasis on their identity as components of several chromatin regulatory complexes. Recent proteomic screens have identified Nups as interacting partners of active and repressive epigenetic machinery, architectural proteins, and DNA replication complexes, providing insight into molecular mechanisms via which Nups regulate gene expression programs. This review summarizes these interactions and discusses their potential functions in the broader framework of nuclear genome organization.
摘要：
核孔复合物（NPC）是嵌入核膜中的大型多组分蛋白质复合物，它们介导核质运输。除了支持运输，核孔隙成分,称为核孔蛋白(Nups)，可以与染色质相互作用并影响基因组功能。Nups的一个子集也可以定位到核内部并在核内结合染色质，提供了一个在运输环境之外研究Nups染色质相关功能的机会。这篇综述集中在这种核内Nups的基因调控功能，特别强调它们作为几种染色质调节复合物成分的身份。最近的蛋白质组学筛选已经确定Nups是活跃和压抑表观遗传机制的相互作用伙伴，建筑蛋白质，和DNA复制复合物，深入了解Nups调控基因表达程序的分子机制。这篇综述总结了这些相互作用，并讨论了它们在核基因组组织的更广泛框架中的潜在功能。
