PDF(Pubmed)
Abstract：
Giardia duodenalis is a gastrointestinal protozoan ubiquitous in nature. It is a confirmed zoonotic pathogen, and cattle are considered a source of giardiasis outbreaks in humans. This study aimed to evaluate the prevalence and multilocus genotype (MLG) of G. duodenalis in dairy cattle in Central Inner Mongolia. This study was based on the small subunit ribosomal RNA (SSU rRNA), glutamate dehydrogenase (gdh), triosephosphate isomerase (tpi), and beta-giardin (bg) genes of G. duodenalis. DNA extraction, polymerase chain reaction (PCR), and sequence analysis were performed on 505 dairy cattle fecal samples collected in 2021 from six sampling sites and four age groups in Central Inner Mongolia to determine the prevalence and MLG distribution of G. duodenalis. The PCR results of SSU rRNA revealed that the overall prevalence of G. duodenalis was 29.5% (149/505) and that the overall prevalence of the diarrhea and nondiarrhea samples was 31.5% (46/146) and 28.5% (103/359), respectively; the difference was not significant (p > 0.05). SSU rRNA sequence analysis revealed that G. duodenalis assemblage E (91.1%, 133/146) was primarily detected and that assemblage A (8.9%, 13/146) was detected in 13 samples. The G. duodenalis-positive samples were PCR amplified and sequenced for gdh, tpi, and bg, from which 38, 47, and 70 amplified sequences were obtained, respectively. A combination of G. duodenalis assemblages A and E were detected in seven samples. Multilocus genotyping yielded 25 different assemblage E MLGs, which formed six subgroups. To the best of our knowledge, this is the first report regarding G. duodenalis infection in dairy cattle in Inner Mongolia, China. This study revealed that Inner Mongolian cattle pose a risk of giardiasis transmission to humans and that the distribution of local cattle G. duodenalis assemblage E MLGs is diverse. The findings of this study can bridge the knowledge gap in the molecular epidemiological investigation of giardiasis in Central Inner Mongolia.
摘要：
十二指肠贾第鞭毛虫是自然界中普遍存在的胃肠道原生动物。这是一种确认的人畜共患病原体，牛被认为是人类贾第鞭毛虫病爆发的源头。本研究旨在评估内蒙古中部地区奶牛十二指肠球藻的患病率和多位点基因型（MLG）。本研究基于小亚基核糖体RNA（SSUrRNA），谷氨酸脱氢酶（gdh），磷酸三糖异构酶（tpi），和β-giardin（bg）基因。DNA提取,聚合酶链反应（PCR），对2021年从内蒙古中部6个采样点和4个年龄组收集的505份奶牛粪便样本进行了序列分析，以确定十二指肠G的患病率和MLG分布。SSUrRNA的PCR结果表明，十二指肠G的总体患病率为29.5％（149/505），腹泻和非腹泻样本的总体患病率为31.5％（46/146）和28.5％（103/359）。差异均不显著(p>0.05)。SSUrRNA序列分析显示，十二指肠G组合E(91.1%，133/146)被主要检测到，组合A(8.9%，13/146)在13个样品中检测到。十二指肠G.阳性样品进行PCR扩增和gdh测序，tpi,而BG,从中获得38、47和70个扩增序列，分别。在七个样品中检测到十二指肠G。组合A和E。多位点基因分型产生了25个不同的组合EMLGs，组成了六个小组。据我们所知,这是关于内蒙古奶牛十二指肠G.daudenalis感染的第一份报告，中国。这项研究表明，内蒙古牛具有将贾第鞭毛虫病传播给人类的风险，并且当地牛G.daudenalis组合EMLGs的分布是多种多样的。本研究结果可弥补内蒙古中部贾第鞭毛虫病分子流行病学调查中的知识空白。
