PDF(Pubmed)
Abstract：
BACKGROUND: Periosteum-mediated bone regeneration (PMBR) is a recognized method for mandibular reconstruction. Despite its unpredictable nature and the limited degree to which it is understood, it does not share the concerns of developmental changes to donor and recipient tissues that other treatment options do. The definitive role of the periosteum in bone regeneration in any mammal remains largely unexplored. The purpose of this study was to identify the genetic determinants of PMBR in mammals through a systematic review.
METHODS: Our search methodology was designed in accordance with the PRISMA (Preferred Reporting Items for Systematic Review and Meta-Analysis) guidelines. We conducted a quality assessment of each publication, and evaluated the differences in gene expression between days 7 and 15.
RESULTS: A total of four studies satisfied the inclusion criteria. The subjects and tissues examined in these studies were Wistar rat calvaria in two studies, mini-pigs in one study, and calves and mice in one study. Three out of the four studies achieved the necessary quality score of ≥ 3. Gene expression analysis showed increased activity of genes responsible for angiogenesis, cytokine activities, and immune-inflammatory responses on day 7. Additionally, genes related to skeletal development and signaling pathways were upregulated on day 15. Conclusions: The results suggest that skeletal morphogenesis is regulated by genes associated with skeletal development, and the gene expression patterns of PMBR may be characterized by specific pathways.
METHODS: Our search methodology was designed in accordance with the PRISMA (Preferred Reporting Items for Systematic Review and Meta-Analysis) guidelines. We conducted a quality assessment of each publication, and evaluated the differences in gene expression between days 7 and 15.
RESULTS: A total of four studies satisfied the inclusion criteria. The subjects and tissues examined in these studies were Wistar rat calvaria in two studies, mini-pigs in one study, and calves and mice in one study. Three out of the four studies achieved the necessary quality score of ≥ 3. Gene expression analysis showed increased activity of genes responsible for angiogenesis, cytokine activities, and immune-inflammatory responses on day 7. Additionally, genes related to skeletal development and signaling pathways were upregulated on day 15. Conclusions: The results suggest that skeletal morphogenesis is regulated by genes associated with skeletal development, and the gene expression patterns of PMBR may be characterized by specific pathways.
摘要：
■骨膜介导的骨再生（PMBR）是下颌骨重建的公认方法。尽管它具有不可预测的性质和对它的理解程度有限，它不像其他治疗方案那样关注供体和受体组织的发育变化。骨膜在任何哺乳动物的骨再生中的决定性作用仍未被探索。本研究的目的是通过系统评价鉴定哺乳动物中PMBR的遗传决定因素。
■：我们的搜索方法是根据PRISMA（系统评价和荟萃分析的首选报告项目）指南设计的。我们对每本刊物进行质量评估,并评估了第7天和第15天之间基因表达的差异。
■共有4项研究满足纳入标准。在这些研究中检查的受试者和组织在两项研究中是Wistar大鼠颅骨，小型猪在一项研究中，在一项研究中，小牛和老鼠。四项研究中有三项达到了必要的质量评分≥3。基因表达分析显示负责血管生成的基因活性增加，细胞因子活性，和第7天的免疫炎症反应。此外,与骨骼发育和信号通路相关的基因在第15天上调.
■s：结果表明，骨骼形态发生受与骨骼发育相关的基因调节，并且PMBR的基因表达模式可以通过特定的途径来表征。
■：我们的搜索方法是根据PRISMA（系统评价和荟萃分析的首选报告项目）指南设计的。我们对每本刊物进行质量评估,并评估了第7天和第15天之间基因表达的差异。
■共有4项研究满足纳入标准。在这些研究中检查的受试者和组织在两项研究中是Wistar大鼠颅骨，小型猪在一项研究中，在一项研究中，小牛和老鼠。四项研究中有三项达到了必要的质量评分≥3。基因表达分析显示负责血管生成的基因活性增加，细胞因子活性，和第7天的免疫炎症反应。此外,与骨骼发育和信号通路相关的基因在第15天上调.
■s：结果表明，骨骼形态发生受与骨骼发育相关的基因调节，并且PMBR的基因表达模式可以通过特定的途径来表征。
