METHODS: Our search methodology was designed in accordance with the PRISMA (Preferred Reporting Items for Systematic Review and Meta-Analysis) guidelines. We conducted a quality assessment of each publication, and evaluated the differences in gene expression between days 7 and 15.
RESULTS: A total of four studies satisfied the inclusion criteria. The subjects and tissues examined in these studies were Wistar rat calvaria in two studies, mini-pigs in one study, and calves and mice in one study. Three out of the four studies achieved the necessary quality score of ≥ 3. Gene expression analysis showed increased activity of genes responsible for angiogenesis, cytokine activities, and immune-inflammatory responses on day 7. Additionally, genes related to skeletal development and signaling pathways were upregulated on day 15. Conclusions: The results suggest that skeletal morphogenesis is regulated by genes associated with skeletal development, and the gene expression patterns of PMBR may be characterized by specific pathways.
■:我们的搜索方法是根据PRISMA(系统评价和荟萃分析的首选报告项目)指南设计的。我们对每本刊物进行质量评估,并评估了第7天和第15天之间基因表达的差异。
■共有4项研究满足纳入标准。在这些研究中检查的受试者和组织在两项研究中是Wistar大鼠颅骨,小型猪在一项研究中,在一项研究中,小牛和老鼠。四项研究中有三项达到了必要的质量评分≥3。基因表达分析显示负责血管生成的基因活性增加,细胞因子活性,和第7天的免疫炎症反应。此外,与骨骼发育和信号通路相关的基因在第15天上调.
■s:结果表明,骨骼形态发生受与骨骼发育相关的基因调节,并且PMBR的基因表达模式可以通过特定的途径来表征。