Abstract：
Endurance exercise can disturb intestinal epithelial integrity, leading to increased systemic indicators of cell injury, hyperpermeability, and pathogenic translocation. However, the interaction between exercise, diet, and gastrointestinal disturbance still warrants exploration. This study examined whether a 6-day dietary intervention influenced perturbations to intestinal epithelial disruption in response to a 25-km race walk. Twenty-eight male race walkers adhered to a high carbohydrate (CHO)/energy diet (65% CHO, energy availability = 40 kcal·kg FFM-1·day-1) for 6 days prior to a Baseline 25-km race walk. Athletes were then split into three subgroups: high CHO/energy diet (n = 10); low-CHO, high-fat diet (LCHF: n = 8; <50 g/day CHO, energy availability = 40 kcal·kg FFM-1·day-1); and low energy availability (n = 10; 65% CHO, energy availability = 15 kcal·kg FFM-1·day-1) for a further 6-day dietary intervention period prior to a second 25-km race walk (Adaptation). During both trials, venous blood was collected pre-, post-, and 1 hr postexercise and analyzed for markers of intestinal epithelial disruption. Intestinal fatty acid-binding protein concentration was significantly higher (twofold increase) in response to exercise during Adaptation compared to Baseline in the LCHF group (p = .001). Similar findings were observed for soluble CD14 (p < .001) and lipopolysaccharide-binding protein (p = .003), where postexercise concentrations were higher (53% and 36%, respectively) during Adaptation than Baseline in LCHF. No differences in high CHO/energy diet or low energy availability were apparent for any blood markers assessed (p > .05). A short-term LCHF diet increased intestinal epithelial cell injury in response to a 25-km race walk. No effect of low energy availability on gastrointestinal injury or symptoms was observed.
摘要：
耐力运动会扰乱肠上皮的完整性，导致细胞损伤的全身指标增加，高渗透性,和致病性易位。然而,运动之间的相互作用，饮食，胃肠道紊乱仍值得探索。这项研究检查了6天的饮食干预是否会影响25公里竞走对肠上皮破坏的扰动。28名男性竞走者坚持高碳水化合物（CHO）/能量饮食（65％CHO，能量可用性=40kcal·kgFFM-1·day-1）在基线25公里竞走之前的6天。然后将运动员分为三个亚组：高CHO/能量饮食（n=10）；低CHO，高脂肪饮食（LCHF：n=8；<50克/天CHO，能量可用性=40千卡·千克FFM-1·天-1)；和低能量可用性(n=10；65%CHO，能量可用性=15kcal·kgFFM-1·day-1），在第二次25公里竞走（适应）之前再进行6天的饮食干预期。在两次审判中，静脉血收集前，post-,和运动后1小时，并分析肠上皮破坏的标志物。与基线相比，LCHF组的肠道脂肪酸结合蛋白浓度显着升高（两倍增加）。对于可溶性CD14（p<.001）和脂多糖结合蛋白（p=.003），运动后的浓度较高(53%和36%，分别)在LCHF的适应期间比基线。对于所评估的任何血液标志物,高CHO/能量饮食或低能量可用性没有明显差异(p>.05)。短期LCHF饮食会增加25公里竞走引起的肠上皮细胞损伤。未观察到低能量可用性对胃肠道损伤或症状的影响。
