Abstract：
To evaluate the influence of two glutaraldehyde-based desensitizers (L: GLUMA Desensitizer, Heraeus Kulzer and G: GLUMA Desensitizer PowerGel) prior to the adhesive procedures on microtensile bond strength (µTBS) to dentin and endogenous enzymatic activity.
Noncarious human third molars (N = 48) were cut to expose middle coronal dentin. Six experimental groups were formed according to the dentin pre-treatment (L or G) and the universal adhesives (IBU - iBond universal, Kulzer or AU - Adhese Universal, Ivoclar Vivadent) used in the self-etch mode (n = 8): 1) L/IBU; 2) G/IBU; 3) IBU; 4) L/AU; 5) G/AU; 6) AU. Specimens were cut into sticks and stressed until failure after 24 h (T0) or 1 yr of aging (T12). Additional 4 teeth were used for in situ zymography evaluation and data were statistically analyzed (α = 0.05).
Dentin pre-treatment, adhesive and aging statistically influenced bond strength and enzymatic activity (P<0.001). AU demonstrated higher bond strength values than IBU (P<0.001). The L resulted in higher bond strength compared to the G and control groups (P<0.001). aging statistically influenced bonding performance, especially when no dentin pre-treatment was performed (P<0.001). In situ zymography revealed that at baseline the control groups exhibited lower interfacial fluorescence compared to the experimental groups, irrespective of the adhesive used (P<0,001). However, after 1 yr of artificial storage, no differences were found among the groups (P>0.05).
Glutharldeadeyde-based products increased bond strength and determined a stabilization of the adhesive interface over time apparently not related to the MMPs inhibition.
The results of this in vitro study suggest that the application of glutaraldehyde-based desensitizers prior to the adhesive procedures when associated with universal adhesives could result in increased bond strength and stabilization of the adhesive interface over time.
Noncarious human third molars (N = 48) were cut to expose middle coronal dentin. Six experimental groups were formed according to the dentin pre-treatment (L or G) and the universal adhesives (IBU - iBond universal, Kulzer or AU - Adhese Universal, Ivoclar Vivadent) used in the self-etch mode (n = 8): 1) L/IBU; 2) G/IBU; 3) IBU; 4) L/AU; 5) G/AU; 6) AU. Specimens were cut into sticks and stressed until failure after 24 h (T0) or 1 yr of aging (T12). Additional 4 teeth were used for in situ zymography evaluation and data were statistically analyzed (α = 0.05).
Dentin pre-treatment, adhesive and aging statistically influenced bond strength and enzymatic activity (P<0.001). AU demonstrated higher bond strength values than IBU (P<0.001). The L resulted in higher bond strength compared to the G and control groups (P<0.001). aging statistically influenced bonding performance, especially when no dentin pre-treatment was performed (P<0.001). In situ zymography revealed that at baseline the control groups exhibited lower interfacial fluorescence compared to the experimental groups, irrespective of the adhesive used (P<0,001). However, after 1 yr of artificial storage, no differences were found among the groups (P>0.05).
Glutharldeadeyde-based products increased bond strength and determined a stabilization of the adhesive interface over time apparently not related to the MMPs inhibition.
The results of this in vitro study suggest that the application of glutaraldehyde-based desensitizers prior to the adhesive procedures when associated with universal adhesives could result in increased bond strength and stabilization of the adhesive interface over time.
摘要：
目的：评估两种基于戊二醛的脱敏剂（L：GLUMA脱敏剂，HeraeusKulzer和G：GLUMA脱敏剂PowerGel）在进行微拉伸粘合强度（µTBS）与牙本质和内源性酶活性的粘合程序之前。
方法：切割非龋齿人类第三磨牙（N=48）以暴露中冠牙本质。根据牙本质预处理（L或G）和通用粘合剂（IBU-iBond通用，Kulzer或AU-AdheseUniversal，IvoclarVivadent）用于自蚀刻模式（n=8）：1）L/IBU；2）G/IBU；3）IBU；4）L/AU；5）G/AU；6）AU。将样品切成棒并加压直到24小时（T0）或1年老化（T12）后失效。另外使用4颗牙齿进行原位酶谱评估，并对数据进行统计学分析（α=0.05）。
结果：牙本质预处理，粘附和老化对粘结强度和酶活性有统计学影响(P<0.001)。AU表现出比IBU更高的粘结强度值(P<0.001)。与G组和对照组相比,L导致更高的粘结强度(P<0.001)。老化在统计上影响粘接性能，特别是当没有进行牙本质预处理时（P<0.001）。原位酶谱显示，在基线时，与实验组相比，对照组表现出更低的界面荧光。无论使用的粘合剂（P<0,001）。然而,人工储存1年后，组间比较差异无统计学意义(P>0.05)。
结论：基于Gluthardeadeyde的产品增加了粘合强度，并确定了粘合剂界面随着时间的稳定，显然与MMPs抑制无关。
结论：这项体外研究的结果表明，当与通用粘合剂结合时，在粘合剂程序之前使用戊二醛基脱敏剂可以提高粘合强度和粘合剂界面的稳定性。
方法：切割非龋齿人类第三磨牙（N=48）以暴露中冠牙本质。根据牙本质预处理（L或G）和通用粘合剂（IBU-iBond通用，Kulzer或AU-AdheseUniversal，IvoclarVivadent）用于自蚀刻模式（n=8）：1）L/IBU；2）G/IBU；3）IBU；4）L/AU；5）G/AU；6）AU。将样品切成棒并加压直到24小时（T0）或1年老化（T12）后失效。另外使用4颗牙齿进行原位酶谱评估，并对数据进行统计学分析（α=0.05）。
结果：牙本质预处理，粘附和老化对粘结强度和酶活性有统计学影响(P<0.001)。AU表现出比IBU更高的粘结强度值(P<0.001)。与G组和对照组相比,L导致更高的粘结强度(P<0.001)。老化在统计上影响粘接性能，特别是当没有进行牙本质预处理时（P<0.001）。原位酶谱显示，在基线时，与实验组相比，对照组表现出更低的界面荧光。无论使用的粘合剂（P<0,001）。然而,人工储存1年后，组间比较差异无统计学意义(P>0.05)。
结论：基于Gluthardeadeyde的产品增加了粘合强度，并确定了粘合剂界面随着时间的稳定，显然与MMPs抑制无关。
结论：这项体外研究的结果表明，当与通用粘合剂结合时，在粘合剂程序之前使用戊二醛基脱敏剂可以提高粘合强度和粘合剂界面的稳定性。
