Abstract：
The transition to renewable energy sources is crucial to ensure a sustainable future. Although the sugar and ethanol industries benefit from this transition, there are untapped opportunities to utilize the waste generated from the sugar and ethanol process chains through two-stage anaerobic digestion (TSAD). This review comprehensively discusses the utilization of various sugarcane-based industrial wastes by TSAD for sequential biohydrogen and methane production. Factors influencing TSAD process performance, including pH, temperature, hydraulic retention time, volatile fatty acids and alkalinity, nutrient imbalance, microbial population, and inhibitors, were discussed in detail. The potential of TSAD to reduce emissions of greenhouse gases is demonstrated. Recent findings, implications, and promising future research related to TSAD, including the integration of meta-omics approaches, gene manipulation and bioaugmentation, and application of artificial intelligence, are highlighted. The review can serve as important literature for the implementation, improvement, and advancements in TSAD research.
摘要：
向可再生能源过渡对于确保可持续的未来至关重要。尽管糖和乙醇行业受益于这一转变，存在通过两阶段厌氧消化(TSAD)利用糖和乙醇工艺链产生的废物的未开发机会。这篇综述全面讨论了TSAD对各种甘蔗基工业废物的利用，用于顺序生产生物氢和甲烷。影响TSAD工艺性能的因素,包括pH值,温度,水力停留时间,挥发性脂肪酸和碱度，营养失衡,微生物种群,和抑制剂，进行了详细讨论。证明了TSAD减少温室气体排放的潜力。最近的发现,含义，以及与TSAD相关的有希望的未来研究，包括元组学方法的整合，基因操作和生物增强，和人工智能的应用，被突出显示。这篇综述可以作为实施的重要文献，改进,以及TSAD研究的进展。
