Abstract：
In the modern world, many people are changing old dietary and lifestyle habits to improve the quality of their living-to treat or just prevent possible diseases. The main goal of this pilot study was to assess the food and lifestyle impact on exhaled breath volatile organic compounds (VOCs) in various population groups. It was done by employing a recently validated portable membrane-inlet mass spectrometer-MIMS. Thus, the obtained results would also represent the additional confirmation for the employment of the new instrument in the breath analysis. The pilot study involved 151 participants across Europe, including people with overweight, obesity, type 2 diabetes mellitus, cardiovascular disease, people with poor-quality diet and professional athletes. Exhaled breath acetone, ethanol, isoprene, and n-pentane levels were determined in samples before the meal, and 120 min after the meal. Obtained basal ppbvvalues were mainly in accordance with previously reported, which confirms that MIMS instrument can be used in the breath analysis. Combining the quantified levels along with the information about the participants\' lifestyle habits collected via questionnaire, an assessment of the food and lifestyle impact was obtained. Notable alteration in examined VOC levels upon meal consumption was detected in more than 70% of all participants, with exception for isoprene, which was affected in about half of participants. Lifestyle parameters impact was examined using statistical analysis of variance (ANOVA) on ranks test. Statistically significant differences in basal breath VOC levels were observed among all examined population groups. Also, n-pentane and ethanol levels significantly differed in people of different ages, as well as acetone levels in people with different physical activity habits. These findings are promising for further, more focused research using MIMS technique in breath analysis.
摘要：
在现代世界,许多人正在改变旧的饮食和生活习惯，以提高他们的生活质量-治疗或预防可能的疾病。这项初步研究的主要目标是评估不同人群的食物和生活方式对呼出气挥发性有机化合物（VOC）的影响。这是通过使用最近验证的便携式膜入口质谱仪-MIMS来完成的。因此，获得的结果也将代表新仪器在呼吸分析中的应用的额外确认。这项试点研究涉及欧洲的151名参与者，包括超重的人,肥胖，2型糖尿病,心血管疾病,饮食质量差和专业运动员的人。呼气丙酮，乙醇,异戊二烯,在餐前测定样品中的正戊烷含量，饭后120分钟。获得的基础ppbv值主要与先前报道的一致，这证实了MIMS仪器可以用于呼吸分析。将量化水平与通过问卷收集的参与者生活习惯信息相结合，对食物和生活方式的影响进行了评估.在所有参与者中，超过70％的人在用餐时检测到VOC水平的显着变化。除了异戊二烯,大约一半的参与者受到了影响。使用等级检验的方差统计分析(ANOVA)检查生活方式参数影响。在所有被检查的人群组中观察到基础呼吸VOC水平的统计学显着差异。此外，正戊烷和乙醇水平在不同年龄的人群中差异显著，以及具有不同身体活动习惯的人的丙酮水平。这些发现有希望进一步，在呼吸分析中使用MIMS技术进行更集中的研究。
