PDF(Pubmed)
Abstract：
Morphological characteristics and DNA sequencing were used to identify plerocercoids of a Schistocephalus sp. infecting slimy sculpin (Cottus cognatus) from northern New Brunswick and plerocercoids of Ligula intestinalis infecting blacknose dace (Rhinichthys atratulus) in Fundy National Park (FNP, New Brunswick). To our knowledge, no previous publications documented either cestode from New Brunswick, Canada. Blacknose dace represent a new host record for L. intestinalis. Identifications were made based on the presence or absence of segmentation and sequencing partial nicotinamide adenine dinucleotide dehydrogenase subunit 1 (ND1; mitochondrial DNA) and/or partial cytochrome c oxidase subunit 1 (COI; mitochondrial DNA). Plerocercoids from blacknose dace in FNP were identified as Ligula intestinalis based on >99% nucleotide identity with COI for this species in the NCBI GenBank database. Plerocercoids in slimy sculpin from northern New Brunswick were identified as a Schistocephalus sp. based on high nucleotide identity with congenerics in the NCBI GenBank database. The absence of GenBank entries with sufficient high percent identity to our specimens, and potential species hybrids in this genus, prevents species-level identification of Schistocephalus sp. plerocercoids currently. The absence of previous documentation of these cestodes might reflect recent environmental change promoting the transmission of these parasites that can modulate host fish behavior, induce sterility of host fishes, and contribute to epizootics.
摘要：
形态学特征和DNA测序用于鉴定血头菌属的类细菌。从新不伦瑞克省北部感染粘质的sculpin（Cottuscognatus），并在芬迪国家公园（FNP，新不伦瑞克省）。据我们所知,以前的出版物没有记录新不伦瑞克省的任何一个节食，加拿大。Blaknosedace代表了肠乳杆菌的新宿主记录。基于是否存在分段进行鉴定,并对部分烟酰胺腺嘌呤二核苷酸脱氢酶亚基1(ND1;线粒体DNA)和/或部分细胞色素c氧化酶亚基1(COI;线粒体DNA)进行测序。在NCBIGenBank数据库中，基于与该物种的COI的>99％核苷酸同一性，将FNP中的backnosedace中的plerocercoids鉴定为Ligulaintestinalis。来自新不伦瑞克省北部的黏糊糊的鳞茎类动物被鉴定为Schistocephalussp。基于NCBIGenBank数据库中与同系物的高核苷酸同一性。缺乏与我们的标本具有足够高百分比同一性的GenBank条目，和这个属中潜在的物种杂种，阻止了对血头菌的物种水平鉴定。plerocercoids目前。以前没有这些昆虫的文献可能反映了最近的环境变化，促进了这些寄生虫的传播，这些寄生虫可以调节宿主鱼的行为，诱导寄主鱼类不育，并为流行病学做出贡献。
