PDF(Pubmed)
Abstract：
UNASSIGNED: there is little data on the genetic determinants of asthma in Cameroon and sub-Saharan Africa, yet the involvement of genetics in the pathogenesis of this disease has been reported in the literature for several years. This study aims to investigate the possible role of MCP-1 2518 for the risk of asthma in Cameroonians.
UNASSIGNED: we performed a case-control study on 30 volunteers suffering from asthma, matched by aged and sex to 30 healthy subjects. We determine the polymorphism of MCP-1 2518 using restriction fragment length polymorphism following Polymerase Chain Reaction (RFLP-PCR). Fisher exact test was used to compare proportions, with a threshold of significance set at 0.05.
UNASSIGNED: the average age of cases was 21±10 years with 17 (56.7%) females. The distribution of the MCP-1-2518 (A>G) gene polymorphism in people with asthma was as follows: 3 for AA, 5 for GG, and 22 for AG. The minor G allele was predominant (90%) in people with asthma. It was significantly associated with asthma whether the genotype was heterozygous AG or homozygous GG (p<0.01).
UNASSIGNED: MCP-1-2518 (A>G) shows an association with asthma in our sample. Future larger studies evaluating several polymorphisms are needed to describe the genetic determinants of asthma in Cameroon and sub-Saharan Africa.
UNASSIGNED: we performed a case-control study on 30 volunteers suffering from asthma, matched by aged and sex to 30 healthy subjects. We determine the polymorphism of MCP-1 2518 using restriction fragment length polymorphism following Polymerase Chain Reaction (RFLP-PCR). Fisher exact test was used to compare proportions, with a threshold of significance set at 0.05.
UNASSIGNED: the average age of cases was 21±10 years with 17 (56.7%) females. The distribution of the MCP-1-2518 (A>G) gene polymorphism in people with asthma was as follows: 3 for AA, 5 for GG, and 22 for AG. The minor G allele was predominant (90%) in people with asthma. It was significantly associated with asthma whether the genotype was heterozygous AG or homozygous GG (p<0.01).
UNASSIGNED: MCP-1-2518 (A>G) shows an association with asthma in our sample. Future larger studies evaluating several polymorphisms are needed to describe the genetic determinants of asthma in Cameroon and sub-Saharan Africa.
摘要：
关于喀麦隆和撒哈拉以南非洲地区哮喘的遗传决定因素的数据很少，然而，遗传学参与这种疾病的发病机制已经在文献中报道了几年。本研究旨在探讨MCP-12518对喀麦隆哮喘风险的可能作用。
■我们对30名哮喘志愿者进行了病例对照研究，按年龄和性别与30名健康受试者相匹配。我们使用聚合酶链反应（RFLP-PCR）后的限制性片段长度多态性确定MCP-12518的多态性。费舍尔精确检验用于比较比例，显著性阈值设置为0.05。
■病例的平均年龄为21±10岁，其中17名（56.7％）女性。MCP-1-2518（A>G）基因多态性在哮喘患者中的分布如下：3为AA，5为GG，22为AG。次要G等位基因在哮喘患者中占主导地位（90％）。无论基因型是杂合AG还是纯合GG，都与哮喘显着相关（p<0.01）。
■MCP-1-2518（A>G）在我们的样本中显示出与哮喘的关联。未来需要评估几种多态性的更大研究来描述喀麦隆和撒哈拉以南非洲地区哮喘的遗传决定因素。
■我们对30名哮喘志愿者进行了病例对照研究，按年龄和性别与30名健康受试者相匹配。我们使用聚合酶链反应（RFLP-PCR）后的限制性片段长度多态性确定MCP-12518的多态性。费舍尔精确检验用于比较比例，显著性阈值设置为0.05。
■病例的平均年龄为21±10岁，其中17名（56.7％）女性。MCP-1-2518（A>G）基因多态性在哮喘患者中的分布如下：3为AA，5为GG，22为AG。次要G等位基因在哮喘患者中占主导地位（90％）。无论基因型是杂合AG还是纯合GG，都与哮喘显着相关（p<0.01）。
■MCP-1-2518（A>G）在我们的样本中显示出与哮喘的关联。未来需要评估几种多态性的更大研究来描述喀麦隆和撒哈拉以南非洲地区哮喘的遗传决定因素。
